Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore

Susan Chalmers, S.T. Caldwell, C Quin, T.A. Prime, A.M. James, Alan Cairns, M.P. Murphy, John McCarron, R.C. Hartley

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
LanguageEnglish
Pages758-761
Number of pages4
JournalJournal of the American Chemical Society
Volume134
Issue number2
DOIs
Publication statusPublished - 2012

Fingerprint

Mitochondria
2,4-Dinitrophenol
Photolysis
Depolarization
Ultraviolet Rays
Ultraviolet radiation
Cations
Positive ions
Irradiation
Tissue
Molecules

Keywords

  • mitochondria
  • photoactivated protonophore
  • pharmacophores
  • mitochondrion

Cite this

@article{027c5fe2886f429c861a8cf1ba325099,
title = "Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore",
abstract = "Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.",
keywords = "mitochondria , photoactivated protonophore , pharmacophores , mitochondrion",
author = "Susan Chalmers and S.T. Caldwell and C Quin and T.A. Prime and A.M. James and Alan Cairns and M.P. Murphy and John McCarron and R.C. Hartley",
year = "2012",
doi = "10.1021/ja2077922",
language = "English",
volume = "134",
pages = "758--761",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "2",

}

Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore. / Chalmers, Susan; Caldwell, S.T.; Quin, C; Prime, T.A.; James, A.M.; Cairns, Alan; Murphy, M.P.; McCarron, John; Hartley, R.C.

In: Journal of the American Chemical Society , Vol. 134, No. 2, 2012, p. 758-761.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore

AU - Chalmers, Susan

AU - Caldwell, S.T.

AU - Quin, C

AU - Prime, T.A.

AU - James, A.M.

AU - Cairns, Alan

AU - Murphy, M.P.

AU - McCarron, John

AU - Hartley, R.C.

PY - 2012

Y1 - 2012

N2 - Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

AB - Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

KW - mitochondria

KW - photoactivated protonophore

KW - pharmacophores

KW - mitochondrion

UR - http://www.scopus.com/inward/record.url?scp=84855960363&partnerID=8YFLogxK

U2 - 10.1021/ja2077922

DO - 10.1021/ja2077922

M3 - Article

VL - 134

SP - 758

EP - 761

JO - Journal of the American Chemical Society

T2 - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 2

ER -