Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
- photoactivated protonophore
Chalmers, S., Caldwell, S. T., Quin, C., Prime, T. A., James, A. M., Cairns, A., ... Hartley, R. C. (2012). Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore. Journal of the American Chemical Society , 134(2), 758-761. https://doi.org/10.1021/ja2077922