Selective solid-phase extraction of amoxicillin and cephalexin from urine samples using a molecularly imprinted polymer

Antoni Beltran, Rosa M. Marce, Peter A. G. Cormack, David C. Sherrington, Francesc Borrull

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In this paper we describe, for the first time, a molecularly imprinted polymer (MIP) for the antibiotic amoxicillin (AMX), synthesised by a noncovalent molecular imprinting approach and used to extract AMX selectively from urine samples. The MIP was applied as a molecularly selective sorbent in molecularly imprinted SPE (MISPE) in an off-line mode, where it showed useful cross-selectivity for a structurally related antibiotic, cephalexin (M). By using a MISPE protocol, the MIP was able to selectively extract both AMX and CFX from 5 mL of water spiked with 10 mg/L with recoveries of 75 and 78% for AMX and CFX, respectively. When applied to real samples (urine) at clinically relevant concentrations, recoveries from 2 mL of human urine spiked with 20 mg/L decreased slightly to 65 and 63% for AMX and CFX, respectively. To demonstrate further the selectivity of the MIP obtained, a comparison with commercially available SPE cartridges was performed. Improvements in the retention of both AMX and CFX on the MIP were obtained relative to the commercially available cartridges, and the MISPE extracts were considerably cleaner, due to molecularly selective analyte binding by the MIP. Copyright © 2010 Thomson Reuters
LanguageEnglish
Pages2868-2874
Number of pages6
JournalJournal of Separation Science
Volume31
Issue number15
DOIs
Publication statusPublished - 19 Aug 2008

Fingerprint

Cephalexin
Amoxicillin
Polymers
Antibiotics
Anti-Bacterial Agents
Recovery
Sorbents
Water

Keywords

  • amoxicillin
  • cephalexin
  • molecularly imprinted polymer
  • solid-phase extraction
  • urine samples

Cite this

Beltran, Antoni ; Marce, Rosa M. ; Cormack, Peter A. G. ; Sherrington, David C. ; Borrull, Francesc. / Selective solid-phase extraction of amoxicillin and cephalexin from urine samples using a molecularly imprinted polymer. In: Journal of Separation Science. 2008 ; Vol. 31, No. 15. pp. 2868-2874.
@article{bdcc7aabda384c13ba245e185a40fdb6,
title = "Selective solid-phase extraction of amoxicillin and cephalexin from urine samples using a molecularly imprinted polymer",
abstract = "In this paper we describe, for the first time, a molecularly imprinted polymer (MIP) for the antibiotic amoxicillin (AMX), synthesised by a noncovalent molecular imprinting approach and used to extract AMX selectively from urine samples. The MIP was applied as a molecularly selective sorbent in molecularly imprinted SPE (MISPE) in an off-line mode, where it showed useful cross-selectivity for a structurally related antibiotic, cephalexin (M). By using a MISPE protocol, the MIP was able to selectively extract both AMX and CFX from 5 mL of water spiked with 10 mg/L with recoveries of 75 and 78{\%} for AMX and CFX, respectively. When applied to real samples (urine) at clinically relevant concentrations, recoveries from 2 mL of human urine spiked with 20 mg/L decreased slightly to 65 and 63{\%} for AMX and CFX, respectively. To demonstrate further the selectivity of the MIP obtained, a comparison with commercially available SPE cartridges was performed. Improvements in the retention of both AMX and CFX on the MIP were obtained relative to the commercially available cartridges, and the MISPE extracts were considerably cleaner, due to molecularly selective analyte binding by the MIP. Copyright {\circledC} 2010 Thomson Reuters",
keywords = "amoxicillin, cephalexin, molecularly imprinted polymer, solid-phase extraction, urine samples",
author = "Antoni Beltran and Marce, {Rosa M.} and Cormack, {Peter A. G.} and Sherrington, {David C.} and Francesc Borrull",
year = "2008",
month = "8",
day = "19",
doi = "10.1002/jssc.200800085",
language = "English",
volume = "31",
pages = "2868--2874",
journal = "Journal of Separation Science",
issn = "1615-9306",
number = "15",

}

Selective solid-phase extraction of amoxicillin and cephalexin from urine samples using a molecularly imprinted polymer. / Beltran, Antoni; Marce, Rosa M.; Cormack, Peter A. G.; Sherrington, David C.; Borrull, Francesc.

In: Journal of Separation Science, Vol. 31, No. 15, 19.08.2008, p. 2868-2874.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Selective solid-phase extraction of amoxicillin and cephalexin from urine samples using a molecularly imprinted polymer

AU - Beltran, Antoni

AU - Marce, Rosa M.

AU - Cormack, Peter A. G.

AU - Sherrington, David C.

AU - Borrull, Francesc

PY - 2008/8/19

Y1 - 2008/8/19

N2 - In this paper we describe, for the first time, a molecularly imprinted polymer (MIP) for the antibiotic amoxicillin (AMX), synthesised by a noncovalent molecular imprinting approach and used to extract AMX selectively from urine samples. The MIP was applied as a molecularly selective sorbent in molecularly imprinted SPE (MISPE) in an off-line mode, where it showed useful cross-selectivity for a structurally related antibiotic, cephalexin (M). By using a MISPE protocol, the MIP was able to selectively extract both AMX and CFX from 5 mL of water spiked with 10 mg/L with recoveries of 75 and 78% for AMX and CFX, respectively. When applied to real samples (urine) at clinically relevant concentrations, recoveries from 2 mL of human urine spiked with 20 mg/L decreased slightly to 65 and 63% for AMX and CFX, respectively. To demonstrate further the selectivity of the MIP obtained, a comparison with commercially available SPE cartridges was performed. Improvements in the retention of both AMX and CFX on the MIP were obtained relative to the commercially available cartridges, and the MISPE extracts were considerably cleaner, due to molecularly selective analyte binding by the MIP. Copyright © 2010 Thomson Reuters

AB - In this paper we describe, for the first time, a molecularly imprinted polymer (MIP) for the antibiotic amoxicillin (AMX), synthesised by a noncovalent molecular imprinting approach and used to extract AMX selectively from urine samples. The MIP was applied as a molecularly selective sorbent in molecularly imprinted SPE (MISPE) in an off-line mode, where it showed useful cross-selectivity for a structurally related antibiotic, cephalexin (M). By using a MISPE protocol, the MIP was able to selectively extract both AMX and CFX from 5 mL of water spiked with 10 mg/L with recoveries of 75 and 78% for AMX and CFX, respectively. When applied to real samples (urine) at clinically relevant concentrations, recoveries from 2 mL of human urine spiked with 20 mg/L decreased slightly to 65 and 63% for AMX and CFX, respectively. To demonstrate further the selectivity of the MIP obtained, a comparison with commercially available SPE cartridges was performed. Improvements in the retention of both AMX and CFX on the MIP were obtained relative to the commercially available cartridges, and the MISPE extracts were considerably cleaner, due to molecularly selective analyte binding by the MIP. Copyright © 2010 Thomson Reuters

KW - amoxicillin

KW - cephalexin

KW - molecularly imprinted polymer

KW - solid-phase extraction

KW - urine samples

U2 - 10.1002/jssc.200800085

DO - 10.1002/jssc.200800085

M3 - Article

VL - 31

SP - 2868

EP - 2874

JO - Journal of Separation Science

T2 - Journal of Separation Science

JF - Journal of Separation Science

SN - 1615-9306

IS - 15

ER -