Selective DNA-binding by designed bisbenzamidine-homeodomain chimeras

Jesús Mosquera, Jéssica Rodríguez, M. Eugenio Vázquez, José L. Mascareñas

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor-groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction. Groovy attraction: Conjugates of the helix 3 region of a Q50K engrailed homeodomain with bisbenzamidine minor-groove DNA binders failed to bind DNA. However, modifications that increase the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction.

Original languageEnglish
Pages (from-to)1092-1095
Number of pages4
JournalChembiochem
Volume15
Issue number8
DOIs
Publication statusPublished - 26 May 2014

Keywords

  • DNA recognition
  • molecular recognition
  • oligonucleotides
  • peptides
  • supramolecular chemistry

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