Selective DNA-binding by designed bisbenzamidine-homeodomain chimeras

Jesús Mosquera; Jéssica Rodríguez; M. Eugenio Vázquez; José L. Mascareñas

doi:10.1002/cbic.201400079

Selective DNA-binding by designed bisbenzamidine-homeodomain chimeras

Jesús Mosquera, Jéssica Rodríguez, M. Eugenio Vázquez^*, José L. Mascareñas

^*Corresponding author for this work

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor-groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction. Groovy attraction: Conjugates of the helix 3 region of a Q50K engrailed homeodomain with bisbenzamidine minor-groove DNA binders failed to bind DNA. However, modifications that increase the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction.

Original language	English
Pages (from-to)	1092-1095
Number of pages	4
Journal	Chembiochem
Volume	15
Issue number	8
DOIs	https://doi.org/10.1002/cbic.201400079
Publication status	Published - 26 May 2014

Keywords

DNA recognition
molecular recognition
oligonucleotides
peptides
supramolecular chemistry

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10.1002/cbic.201400079

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abstract = "We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor-groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction. Groovy attraction: Conjugates of the helix 3 region of a Q50K engrailed homeodomain with bisbenzamidine minor-groove DNA binders failed to bind DNA. However, modifications that increase the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction.",

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AU - Mosquera, Jesús

AU - Rodríguez, Jéssica

AU - Vázquez, M. Eugenio

AU - Mascareñas, José L.

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AB - We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor-groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction. Groovy attraction: Conjugates of the helix 3 region of a Q50K engrailed homeodomain with bisbenzamidine minor-groove DNA binders failed to bind DNA. However, modifications that increase the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction.

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Selective DNA-binding by designed bisbenzamidine-homeodomain chimeras

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Keywords

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