Selective detection of deoxyribonucleic acid at ultralow concentrations by SERRS

D. Graham, W.E. Smith, A.M.T. Linacre, C.H. Munro, N.D. Watson, P.C. White

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Genetic research programs such as the human genome project have demonstrated the need for selective detection of nucleic acids at ultralow concentrations (McKusik, V. A. FASEB J. 1991, 5, 12-20, Cui, X.; et al, Proc, Natl, Acad, Sci, U.S.A. 1989, 89, 9389-9393). Current detection techniques require amplification of the genetic material by the polymerase chain reaction (PCR) and detection of the amplified product by fluorescence, A new reliable method for DNA detection has been developed based on adsorption of DNA on colloidal silver and subsequent signal detection using surface-enhanced resonance Raman scattering (SERRS), It uses specifically designed primers and can be applied directly to genetic analysis, Improved surface and aggregation chemistry, utilizing modified DNA and a novel aggregating agent, has resulted in acceptable reproducibility for biological detection, DNA was detected down to 8 x 10(-13) M, which equates to less than one molecule in the interrogation volume at any one time, and an RSD of 10% was obtained, The increased sensitivity compared to fluorescence circumvents the need for an amplification step, and much greater selectivity is obtained due to the sharp vibrational spectra observed, thus reducing the need for separation procedures. The method is potentially sensitive enough to eliminate the need for time-consuming amplification steps and provide a new dimension to labeling chemistry and multiplex analysis of DNA.
LanguageEnglish
Pages4703-4707
Number of pages4
JournalAnalytical Chemistry
Volume69
Issue number22
DOIs
Publication statusPublished - 15 Nov 1997

Fingerprint

Raman scattering
DNA
Amplification
Fluorescence
Polymerase chain reaction
Signal detection
Surface analysis
Vibrational spectra
Labeling
Nucleic Acids
Agglomeration
Genes
Adsorption
Molecules

Keywords

  • surface-enhanced raman
  • colloidal silver particles
  • nucleic-acids
  • scattering
  • spectroscopy
  • DNA
  • adsorption
  • electrode
  • pyridine
  • spectra

Cite this

Graham, D. ; Smith, W.E. ; Linacre, A.M.T. ; Munro, C.H. ; Watson, N.D. ; White, P.C. / Selective detection of deoxyribonucleic acid at ultralow concentrations by SERRS. In: Analytical Chemistry. 1997 ; Vol. 69, No. 22. pp. 4703-4707.
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Selective detection of deoxyribonucleic acid at ultralow concentrations by SERRS. / Graham, D.; Smith, W.E.; Linacre, A.M.T.; Munro, C.H.; Watson, N.D.; White, P.C.

In: Analytical Chemistry, Vol. 69, No. 22, 15.11.1997, p. 4703-4707.

Research output: Contribution to journalArticle

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KW - surface-enhanced raman

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KW - adsorption

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