Selective cytotoxicity of Portuguese Propolis ethyl acetate fraction towards renal cancer cells

Ana Sofia Freitas; Marta Costa; Olívia Pontes; Veronique Seidel; Fernanda Proença; Susana M. Cardoso; Rui Oliveira; Fátima Baltazar; Cristina Almeida-Aguiar

doi:10.3390/molecules27134001

Selective cytotoxicity of Portuguese Propolis ethyl acetate fraction towards renal cancer cells

Ana Sofia Freitas, Marta Costa, Olívia Pontes, Veronique Seidel, Fernanda Proença, Susana M. Cardoso, Rui Oliveira, Fátima Baltazar^*, Cristina Almeida-Aguiar

^*Corresponding author for this work

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (n-hexane, ethyl acetate, n-butanol and water) and three subfractions (P1–P3), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC₅₀ = 0.162 µg mL⁻¹ ) and 786-O (IC₅₀ = 0.271 µg mL⁻¹ ) cells. With similar toxicity against 786-O, P1 (IC₅₀ = 3.8 µg mL⁻¹ ) and P3 (IC₅₀ = 3.1 µg mL⁻¹ ) exhibited greater effect when combined (IC₅₀ = 2.5 µg mL⁻¹ ). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.

Original language	English
Article number	4001
Number of pages	13
Journal	Molecules
Volume	27
Issue number	13
Early online date	22 Jun 2022
DOIs	https://doi.org/10.3390/molecules27134001
Publication status	Published - 22 Jun 2022

Funding

Funding: Ana Freitas acknowledge the financial support provided by national funds through FCT-Portuguese Foundation for Science and Technology (PD/BD/128276/2017), under the Doctoral Programme “Agricultural Production Chains–from fork to farm” (PD/00122/2012) and from the European Social Funds and the Regional Operational Programme Norte 2020. This study was also supported by CITAB research unit (UIDB/04033/2020) and by the “Contrato-Programa” UIDB/04050/2020 funded by national funds through the FCT I.P. An acknowledge also to the University of Aveiro and to Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES) for financial support to the associated laboratory LAQV-REQUIMTE (project reference UIDB/50006/2020), through national funds and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER), within the PT2020 Partnership Agreement. We also acknowledge the financial support from University of Minho, Fundação para a Ciência e a Tecnologia (FCT) and FEDERCOMPETE for financial support through Centro de Química (UID/QUI/00686/2013 and UID/QUI/0686/2016), for the PhD grant awarded to Olívia Pontes (SFRH/BD/128850/2017). The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network (RNRMN) and was purchased within the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT. This work has been funded by National funds, through the Foundation for Science and Technology (FCT)-project UIDB/50026/2020 and UIDP/50026/2020.

Keywords

cytotoxic activity
fractionation
pectolinarigenin
phenolic compounds
propolis
renal cell carcinoma

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

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title = "Selective cytotoxicity of Portuguese Propolis ethyl acetate fraction towards renal cancer cells",

abstract = "Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (n-hexane, ethyl acetate, n-butanol and water) and three subfractions (P1–P3), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC50 = 0.162 µg mL−1 ) and 786-O (IC50 = 0.271 µg mL−1 ) cells. With similar toxicity against 786-O, P1 (IC50 = 3.8 µg mL−1 ) and P3 (IC50 = 3.1 µg mL−1 ) exhibited greater effect when combined (IC50 = 2.5 µg mL−1 ). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.",

keywords = "cytotoxic activity, fractionation, pectolinarigenin, phenolic compounds, propolis, renal cell carcinoma",

author = "Freitas, {Ana Sofia} and Marta Costa and Ol{\'i}via Pontes and Veronique Seidel and Fernanda Proen{\c c}a and Cardoso, {Susana M.} and Rui Oliveira and F{\'a}tima Baltazar and Cristina Almeida-Aguiar",

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T1 - Selective cytotoxicity of Portuguese Propolis ethyl acetate fraction towards renal cancer cells

AU - Freitas, Ana Sofia

AU - Costa, Marta

AU - Pontes, Olívia

AU - Seidel, Veronique

AU - Proença, Fernanda

AU - Cardoso, Susana M.

AU - Oliveira, Rui

AU - Baltazar, Fátima

AU - Almeida-Aguiar, Cristina

PY - 2022/6/22

Y1 - 2022/6/22

N2 - Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (n-hexane, ethyl acetate, n-butanol and water) and three subfractions (P1–P3), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC50 = 0.162 µg mL−1 ) and 786-O (IC50 = 0.271 µg mL−1 ) cells. With similar toxicity against 786-O, P1 (IC50 = 3.8 µg mL−1 ) and P3 (IC50 = 3.1 µg mL−1 ) exhibited greater effect when combined (IC50 = 2.5 µg mL−1 ). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.

AB - Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (n-hexane, ethyl acetate, n-butanol and water) and three subfractions (P1–P3), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC50 = 0.162 µg mL−1 ) and 786-O (IC50 = 0.271 µg mL−1 ) cells. With similar toxicity against 786-O, P1 (IC50 = 3.8 µg mL−1 ) and P3 (IC50 = 3.1 µg mL−1 ) exhibited greater effect when combined (IC50 = 2.5 µg mL−1 ). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.

KW - cytotoxic activity

KW - fractionation

KW - pectolinarigenin

KW - phenolic compounds

KW - propolis

KW - renal cell carcinoma

U2 - 10.3390/molecules27134001

DO - 10.3390/molecules27134001

M3 - Article

AN - SCOPUS:85133193832

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

IS - 13

M1 - 4001

ER -

Selective cytotoxicity of Portuguese Propolis ethyl acetate fraction towards renal cancer cells

Abstract

Funding

Keywords

UN SDGs

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