Secretion of interleukin-10 or interleukin-12 by LPS-activated dendritic cells is critically dependent on time of stimulus relative to initiation of purified DC culture

Hui-Rong Jiang, Elizabeth Muckersie, Marie Robertson, Heping Xu, Janet Liversidge, John V. Forrester

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Dendritic cells (DC) are key regulators of adaptive immunity with the potential to induce T cell activation/immunity or T cell suppression/tolerance. DC are themselves induced by "maturation" signals such as bacterial lipopolysaccharide (LPS). We demonstrate here that LPS can stimulate DC to display similar maturation phenotypes but to differentiate toward an interleukin (IL)-10(high)- or IL-12(high)-secretor profile depending on the timing of maturation signal induction. Immediate/early administration of LPS induced purified bone marrow-derived DC (BMDC) to differentiate as IL-10(high)IL-12(low)-secreting cells, termed early DC (eDC). Conversely, delayed administration of LPS altered the DC cytokine profile to IL-10(low)IL-12(high), termed later DC (lDC). The presence of IL-4 enhanced the yield and maturation of BMDC but inhibited LPS-induced IL-10 production by eDC. In contrast, interferon-gamma reduced the yield of DC but promoted the level of LPS-induced IL-10 production by lDC. Our data provide new evidence that ex vivo manipulation and the cytokine environment regulate DC maturation status and cytokine-secretor phenotype with implications for the control of T cell differentiation and function via DC-based immunotherapeutic strategies.

LanguageEnglish
Pages978-985
Number of pages8
JournalJournal of Leukocyte Biology
Volume72
Issue number5
DOIs
Publication statusPublished - 30 Nov 2002

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Interleukin-12
Interleukin-10
Dendritic Cells
Lipopolysaccharides
Cell Culture Techniques
Cytokines
T-Lymphocytes
Bone Marrow
Phenotype
Adaptive Immunity
Interleukin-4
Interferon-gamma
Cell Differentiation
Immunity

Keywords

  • animals
  • bone marrow cells/immunology
  • CD11c antigen/analysis
  • cell culture techniques
  • cell differentiation
  • cells, cultured
  • dendritic cells/cytology
  • immunophenotyping
  • interferon-gamma/pharmacology
  • interleukin-10/biosynthesis
  • interleukin-12/biosynthesis
  • interleukin-4/pharmacology
  • kinetics
  • lipopolysaccharides/pharmacology
  • lymphocyte activation
  • lymphocyte culture test, mixed
  • male
  • mice
  • signal transduction
  • T-lymphocytes/immunology

Cite this

Jiang, Hui-Rong ; Muckersie, Elizabeth ; Robertson, Marie ; Xu, Heping ; Liversidge, Janet ; Forrester, John V. / Secretion of interleukin-10 or interleukin-12 by LPS-activated dendritic cells is critically dependent on time of stimulus relative to initiation of purified DC culture. In: Journal of Leukocyte Biology . 2002 ; Vol. 72, No. 5. pp. 978-985.
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abstract = "Dendritic cells (DC) are key regulators of adaptive immunity with the potential to induce T cell activation/immunity or T cell suppression/tolerance. DC are themselves induced by {"}maturation{"} signals such as bacterial lipopolysaccharide (LPS). We demonstrate here that LPS can stimulate DC to display similar maturation phenotypes but to differentiate toward an interleukin (IL)-10(high)- or IL-12(high)-secretor profile depending on the timing of maturation signal induction. Immediate/early administration of LPS induced purified bone marrow-derived DC (BMDC) to differentiate as IL-10(high)IL-12(low)-secreting cells, termed early DC (eDC). Conversely, delayed administration of LPS altered the DC cytokine profile to IL-10(low)IL-12(high), termed later DC (lDC). The presence of IL-4 enhanced the yield and maturation of BMDC but inhibited LPS-induced IL-10 production by eDC. In contrast, interferon-gamma reduced the yield of DC but promoted the level of LPS-induced IL-10 production by lDC. Our data provide new evidence that ex vivo manipulation and the cytokine environment regulate DC maturation status and cytokine-secretor phenotype with implications for the control of T cell differentiation and function via DC-based immunotherapeutic strategies.",
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Secretion of interleukin-10 or interleukin-12 by LPS-activated dendritic cells is critically dependent on time of stimulus relative to initiation of purified DC culture. / Jiang, Hui-Rong; Muckersie, Elizabeth; Robertson, Marie; Xu, Heping; Liversidge, Janet; Forrester, John V.

In: Journal of Leukocyte Biology , Vol. 72, No. 5, 30.11.2002, p. 978-985.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Secretion of interleukin-10 or interleukin-12 by LPS-activated dendritic cells is critically dependent on time of stimulus relative to initiation of purified DC culture

AU - Jiang, Hui-Rong

AU - Muckersie, Elizabeth

AU - Robertson, Marie

AU - Xu, Heping

AU - Liversidge, Janet

AU - Forrester, John V.

PY - 2002/11/30

Y1 - 2002/11/30

N2 - Dendritic cells (DC) are key regulators of adaptive immunity with the potential to induce T cell activation/immunity or T cell suppression/tolerance. DC are themselves induced by "maturation" signals such as bacterial lipopolysaccharide (LPS). We demonstrate here that LPS can stimulate DC to display similar maturation phenotypes but to differentiate toward an interleukin (IL)-10(high)- or IL-12(high)-secretor profile depending on the timing of maturation signal induction. Immediate/early administration of LPS induced purified bone marrow-derived DC (BMDC) to differentiate as IL-10(high)IL-12(low)-secreting cells, termed early DC (eDC). Conversely, delayed administration of LPS altered the DC cytokine profile to IL-10(low)IL-12(high), termed later DC (lDC). The presence of IL-4 enhanced the yield and maturation of BMDC but inhibited LPS-induced IL-10 production by eDC. In contrast, interferon-gamma reduced the yield of DC but promoted the level of LPS-induced IL-10 production by lDC. Our data provide new evidence that ex vivo manipulation and the cytokine environment regulate DC maturation status and cytokine-secretor phenotype with implications for the control of T cell differentiation and function via DC-based immunotherapeutic strategies.

AB - Dendritic cells (DC) are key regulators of adaptive immunity with the potential to induce T cell activation/immunity or T cell suppression/tolerance. DC are themselves induced by "maturation" signals such as bacterial lipopolysaccharide (LPS). We demonstrate here that LPS can stimulate DC to display similar maturation phenotypes but to differentiate toward an interleukin (IL)-10(high)- or IL-12(high)-secretor profile depending on the timing of maturation signal induction. Immediate/early administration of LPS induced purified bone marrow-derived DC (BMDC) to differentiate as IL-10(high)IL-12(low)-secreting cells, termed early DC (eDC). Conversely, delayed administration of LPS altered the DC cytokine profile to IL-10(low)IL-12(high), termed later DC (lDC). The presence of IL-4 enhanced the yield and maturation of BMDC but inhibited LPS-induced IL-10 production by eDC. In contrast, interferon-gamma reduced the yield of DC but promoted the level of LPS-induced IL-10 production by lDC. Our data provide new evidence that ex vivo manipulation and the cytokine environment regulate DC maturation status and cytokine-secretor phenotype with implications for the control of T cell differentiation and function via DC-based immunotherapeutic strategies.

KW - animals

KW - bone marrow cells/immunology

KW - CD11c antigen/analysis

KW - cell culture techniques

KW - cell differentiation

KW - cells, cultured

KW - dendritic cells/cytology

KW - immunophenotyping

KW - interferon-gamma/pharmacology

KW - interleukin-10/biosynthesis

KW - interleukin-12/biosynthesis

KW - interleukin-4/pharmacology

KW - kinetics

KW - lipopolysaccharides/pharmacology

KW - lymphocyte activation

KW - lymphocyte culture test, mixed

KW - male

KW - mice

KW - signal transduction

KW - T-lymphocytes/immunology

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DO - 10.1189/jlb.72.5.978

M3 - Article

VL - 72

SP - 978

EP - 985

JO - Journal of Leukocyte Biology

T2 - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 5

ER -