TY - JOUR
T1 - Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy
AU - Bonnier, Franck
AU - Brachet, Guillaume
AU - Duong, Romain
AU - Sojinrin, Tobiloba
AU - Respaud, Renaud
AU - Aubrey, Nicolas
AU - Baker, Matthew J.
AU - Byrne, Hugh J.
AU - Chourpa, Igor
N1 - This is the peer reviewed version of the following article: Bonnier, F., Brachet, G., Duong, R., Sojinrin, T., Respaud, R., Aubrey, N., ... Chourpa, I. (2016). Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy. Journal of Biophotonics, 1-16, which has been published in final form at 10.1002/jbio.201600015. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
PY - 2016/8/10
Y1 - 2016/8/10
N2 - Vibrational spectroscopic techniques can detect small variations in molecular content, linked with disease, showing promise for screening and early diagnosis. Biological fluids, particularly blood serum, are potentially valuable for diagnosis purposes. The so-called Low Molecular Weight Fraction (LMWF) contains the associated peptidome and metabolome and has been identified as potentially the most relevant molecular population for disease-associated biomarker research. Although vibrational spectroscopy can deliver a specific chemical fingerprint of the samples, the High Molecular Weight Fraction (HMWF), composed of the most abundant serum proteins, strongly dominates the response and ultimately makes the detection of minor spectral variations a challenging task. Spectroscopic detection of potential serum biomarkers present at relatively low concentrations can be improved using pre-analytical depletion of the HMWF. In the present study, human serum fractionation by centrifugal filtration was used prior to analysis by Attenuated Total Reflection infrared spectroscopy. Using a model sample based on glycine spiked serum, it is demonstrated that the screening of the LMWF can be applied to quantify blinded concentrations up to 50 times lower. Moreover, the approach is easily transferable to different bodily fluids which would support the development of more efficient and suitable clinical protocols exploring vibrational spectroscopy based ex-vivo diagnostic tools.
AB - Vibrational spectroscopic techniques can detect small variations in molecular content, linked with disease, showing promise for screening and early diagnosis. Biological fluids, particularly blood serum, are potentially valuable for diagnosis purposes. The so-called Low Molecular Weight Fraction (LMWF) contains the associated peptidome and metabolome and has been identified as potentially the most relevant molecular population for disease-associated biomarker research. Although vibrational spectroscopy can deliver a specific chemical fingerprint of the samples, the High Molecular Weight Fraction (HMWF), composed of the most abundant serum proteins, strongly dominates the response and ultimately makes the detection of minor spectral variations a challenging task. Spectroscopic detection of potential serum biomarkers present at relatively low concentrations can be improved using pre-analytical depletion of the HMWF. In the present study, human serum fractionation by centrifugal filtration was used prior to analysis by Attenuated Total Reflection infrared spectroscopy. Using a model sample based on glycine spiked serum, it is demonstrated that the screening of the LMWF can be applied to quantify blinded concentrations up to 50 times lower. Moreover, the approach is easily transferable to different bodily fluids which would support the development of more efficient and suitable clinical protocols exploring vibrational spectroscopy based ex-vivo diagnostic tools.
KW - human serum
KW - centrifugal filtration
KW - IR spectroscopy
KW - attenuated total reflection
KW - principal component analysis
KW - low molecular weight fraction
U2 - 10.1002/jbio.201600015
DO - 10.1002/jbio.201600015
M3 - Article
SN - 1864-063X
SP - 1
EP - 16
JO - Journal of Biophotonics
JF - Journal of Biophotonics
ER -