SAM composition and electrode roughness affect performance of a DNA biosensor for antibiotic resistance

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2 Citations (Scopus)

Abstract

Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance.
LanguageEnglish
Article number22
Number of pages12
JournalBiosensors
Volume9
Issue number1
DOIs
Publication statusPublished - 7 Feb 2019

Fingerprint

Biosensing Techniques
Self assembled monolayers
Antibiotics
Microbial Drug Resistance
Biosensors
Electrodes
DNA
Surface roughness
Anti-Bacterial Agents
Chemical analysis
Assays
Point-of-Care Systems
Electrochemical Techniques
Communicable Diseases
Genes
Costs and Cost Analysis
Testing
Therapeutics
Costs

Keywords

  • electrochemical biosensor
  • DNA detection
  • point-of-care diagnostics
  • electrochemical impedance spectroscopy
  • self-assembled monolayers (SAMs)

Cite this

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title = "SAM composition and electrode roughness affect performance of a DNA biosensor for antibiotic resistance",
abstract = "Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance.",
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author = "Adrian Butterworth and Elizabeth Blues and Paul Williamson and Cardona, {Milovan Joe} and Louise Gray and Corrigan, {Damion K}",
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KW - electrochemical impedance spectroscopy

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