Salting-in with a salting-out agent: explaining the cation specific effects on the aqueous solubility of amino acids

Luciana I. N. Tome, Simão P Pinho, Miguel Jorge, Jose R. B. Gomes, Joao A. P. Coutinho

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Although the understanding of ion specific effects on the aqueous solubilities of biomolecules is crucial for the development of many areas of biochemistry and life sciences, a consensual and well-supported molecular picture of the phenomena has not yet been established. Mostly, the influence of cations and the nature of the molecular interactions responsible for the reversal of the Hofmeister trend in aqueous solutions of amino acids and proteins are still defectively understood. Aiming at contributing to the understanding of the molecular-level mechanisms governing the cation specific effects on the aqueous solubilities of biocompounds, experimental solubility measurements and classical molecular dynamics simulations were performed for aqueous solutions of three amino acids (alanine, valine, and isoleucine), in the presence of a series of inorganic salts. The evidence gathered suggests that the mechanism by which salting-in inducing cations operate in aqueous solutions of amino acids is different from that of anions, and allows for a novel and consistent molecular description of the effect of the cation on the solubility based on specific interactions of the cations with the negatively charged moieties of the biomolecules.
LanguageEnglish
Pages6116-6128
Number of pages13
JournalJournal of Physical Chemistry B
Volume117
Issue number20
DOIs
Publication statusPublished - 23 May 2013

Fingerprint

amino acids
Cations
Amino acids
solubility
Solubility
Positive ions
Amino Acids
cations
Biomolecules
aqueous solutions
biochemistry
Biochemistry
life sciences
Molecular interactions
Isoleucine
Valine
alanine
molecular interactions
Alanine
Anions

Keywords

  • amino acids
  • salting out agent
  • biomolecule aqueous solubility
  • aqueous solutions

Cite this

Tome, Luciana I. N. ; Pinho, Simão P ; Jorge, Miguel ; Gomes, Jose R. B. ; Coutinho, Joao A. P. / Salting-in with a salting-out agent : explaining the cation specific effects on the aqueous solubility of amino acids. In: Journal of Physical Chemistry B. 2013 ; Vol. 117, No. 20. pp. 6116-6128.
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abstract = "Although the understanding of ion specific effects on the aqueous solubilities of biomolecules is crucial for the development of many areas of biochemistry and life sciences, a consensual and well-supported molecular picture of the phenomena has not yet been established. Mostly, the influence of cations and the nature of the molecular interactions responsible for the reversal of the Hofmeister trend in aqueous solutions of amino acids and proteins are still defectively understood. Aiming at contributing to the understanding of the molecular-level mechanisms governing the cation specific effects on the aqueous solubilities of biocompounds, experimental solubility measurements and classical molecular dynamics simulations were performed for aqueous solutions of three amino acids (alanine, valine, and isoleucine), in the presence of a series of inorganic salts. The evidence gathered suggests that the mechanism by which salting-in inducing cations operate in aqueous solutions of amino acids is different from that of anions, and allows for a novel and consistent molecular description of the effect of the cation on the solubility based on specific interactions of the cations with the negatively charged moieties of the biomolecules.",
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Salting-in with a salting-out agent : explaining the cation specific effects on the aqueous solubility of amino acids. / Tome, Luciana I. N.; Pinho, Simão P; Jorge, Miguel; Gomes, Jose R. B.; Coutinho, Joao A. P.

In: Journal of Physical Chemistry B, Vol. 117, No. 20, 23.05.2013, p. 6116-6128.

Research output: Contribution to journalArticle

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