S-acylation regulates the trafficking and stability of the unconventional Q-SNARE STX19

Khamal K. Ampah, Jennifer Greaves, Amber S. M. Shun-Shion, Asral W. B. A. Asnawi, Jessica A. Lidster, Luke H. Chamberlain, Mark O. Collins, Andrew A. Peden

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

STX19 is an unusual Qa-SNARE as it lacks a C-terminal transmembrane domain. However, it is efficiently targeted to post-Golgi membranes. We have set out to determine the intracellular localisation of endogenous STX19 and elucidate the mechanism by which it is targeted to membranes. We have found that a pool of STX19 is localised to tubular recycling endosomes where it co-localises with MICAL-L1 and Rab8. Using a combination of genetic, biochemical and cell based approaches we have identified that STX19 is S-acylated at its C-terminus and is a substrate for several Golgi localised S-acyltransferases, suggesting that STX19 is initially S-acylated at the Golgi before trafficking to the plasma membrane and endosomes. Surprisingly, we have found that S-acylation is the key determinant in targeting STX19 to tubular recycling endosomes, suggesting that S-acylation may play a general role in directing proteins to this compartment. In addition, S-acylation also protects STX19 from proteosomal degradation indicating that S-acylation regulates the function of STX19 at multiple levels.
LanguageEnglish
Number of pages48
JournalJournal of Cell Science
Early online date25 Sep 2018
DOIs
Publication statusE-pub ahead of print - 25 Sep 2018

Fingerprint

Q-SNARE Proteins
Acylation
Endosomes
Qa-SNARE Proteins
Acyltransferases
Membranes
Molecular Biology
Cell Membrane
Proteins

Keywords

  • SNARE
  • S-acylation
  • palmitoylation
  • STX19
  • MICAL-L1
  • Rab8

Cite this

Ampah, K. K., Greaves, J., Shun-Shion, A. S. M., Asnawi, A. W. B. A., Lidster, J. A., Chamberlain, L. H., ... Peden, A. A. (2018). S-acylation regulates the trafficking and stability of the unconventional Q-SNARE STX19. Journal of Cell Science. https://doi.org/10.1242/jcs.212498
Ampah, Khamal K. ; Greaves, Jennifer ; Shun-Shion, Amber S. M. ; Asnawi, Asral W. B. A. ; Lidster, Jessica A. ; Chamberlain, Luke H. ; Collins, Mark O. ; Peden, Andrew A. / S-acylation regulates the trafficking and stability of the unconventional Q-SNARE STX19. In: Journal of Cell Science. 2018.
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S-acylation regulates the trafficking and stability of the unconventional Q-SNARE STX19. / Ampah, Khamal K.; Greaves, Jennifer; Shun-Shion, Amber S. M.; Asnawi, Asral W. B. A.; Lidster, Jessica A.; Chamberlain, Luke H.; Collins, Mark O.; Peden, Andrew A.

In: Journal of Cell Science, 25.09.2018.

Research output: Contribution to journalArticle

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AU - Greaves, Jennifer

AU - Shun-Shion, Amber S. M.

AU - Asnawi, Asral W. B. A.

AU - Lidster, Jessica A.

AU - Chamberlain, Luke H.

AU - Collins, Mark O.

AU - Peden, Andrew A.

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