Ruthenation of non-stacked guanines in DNA G-quadruplex structures: enhancement of c-MYC expression

Jéssica Rodríguez; Jesús Mosquera; José R. Couceiro; M. Eugenio Vázquez; José L. Mascareñas

doi:10.1002/anie.201607965

Ruthenation of non-stacked guanines in DNA G-quadruplex structures: enhancement of c-MYC expression

Jéssica Rodríguez, Jesús Mosquera, José R. Couceiro, M. Eugenio Vázquez^*, José L. Mascareñas

^*Corresponding author for this work

Pure And Applied Chemistry

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Abstract

Guanine quadruplexes (GQs) are compact four-stranded DNA structures that play a key role in the control of a variety of biological processes, including gene transcription. Bulky ruthenium complexes featuring a bipyridine, a terpyridine, and one exchangeable ligand ([Ru(terpy)(bpy)X]ⁿ⁺) are able to metalate exposed guanines present in the GQ of the c-MYC promoter region that are not involved in quadruplex base pairing. qRT-PCR and western-blot experiments indicated that the complexes promote a remarkable increase in the expression of this oncogene. We also show that exchangeable thioether ligands (X=RSR′, Met) allow regulation of the metalating activity of the complex with visible light.

Original language	English
Pages (from-to)	15615-15618
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	55
Issue number	50
Early online date	17 Nov 2016
DOIs	https://doi.org/10.1002/anie.201607965
Publication status	Published - 12 Dec 2016

Keywords

bioinorganic chemistry
DNA damage
metalation
photochemistry
ruthenium

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Rodríguez-etal-ACIE2016-Ruthenation-non-stacked-guanines-DNA-G-quadruplex-structuresFinal published version, 991 KBLicence: CC BY-NC-ND 4.0

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abstract = "Guanine quadruplexes (GQs) are compact four-stranded DNA structures that play a key role in the control of a variety of biological processes, including gene transcription. Bulky ruthenium complexes featuring a bipyridine, a terpyridine, and one exchangeable ligand ([Ru(terpy)(bpy)X]n+) are able to metalate exposed guanines present in the GQ of the c-MYC promoter region that are not involved in quadruplex base pairing. qRT-PCR and western-blot experiments indicated that the complexes promote a remarkable increase in the expression of this oncogene. We also show that exchangeable thioether ligands (X=RSR′, Met) allow regulation of the metalating activity of the complex with visible light.",

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AU - Rodríguez, Jéssica

AU - Mosquera, Jesús

AU - Couceiro, José R.

AU - Vázquez, M. Eugenio

AU - Mascareñas, José L.

PY - 2016/12/12

Y1 - 2016/12/12

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KW - bioinorganic chemistry

KW - DNA damage

KW - metalation

KW - photochemistry

KW - ruthenium

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IS - 50

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Ruthenation of non-stacked guanines in DNA G-quadruplex structures: enhancement of c-MYC expression

Abstract

Keywords

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