Ruthenation of non-stacked guanines in DNA G-quadruplex structures: enhancement of c-MYC expression

Jéssica Rodríguez, Jesús Mosquera, José R. Couceiro, M. Eugenio Vázquez, José L. Mascareñas

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Guanine quadruplexes (GQs) are compact four-stranded DNA structures that play a key role in the control of a variety of biological processes, including gene transcription. Bulky ruthenium complexes featuring a bipyridine, a terpyridine, and one exchangeable ligand ([Ru(terpy)(bpy)X]n+) are able to metalate exposed guanines present in the GQ of the c-MYC promoter region that are not involved in quadruplex base pairing. qRT-PCR and western-blot experiments indicated that the complexes promote a remarkable increase in the expression of this oncogene. We also show that exchangeable thioether ligands (X=RSR′, Met) allow regulation of the metalating activity of the complex with visible light.

Original languageEnglish
Pages (from-to)15615-15618
Number of pages4
JournalAngewandte Chemie - International Edition
Volume55
Issue number50
Early online date17 Nov 2016
DOIs
Publication statusPublished - 12 Dec 2016

Keywords

  • bioinorganic chemistry
  • DNA damage
  • metalation
  • photochemistry
  • ruthenium

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