Role of endosomal Fab GTPases in cytokinesis

Xinzi Yu, Rytis Prekeris, Gwyn W. Gould

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Completion of cytokinesis requires Rab 11-dependent membrane trafficking events to deliver new membrane to the furrow and for abscission. Many Rabs have overlapping endosomal distributions, hence, we examined whether these Rabs also function in cytokinesis. Analysis of the distribution of Rabs 4, 5, 7, 8, 9, 11, 21, and 22 revealed that only Rab 11 was enriched within the furrow of cells in telophase or present within the midbody. By contrast, Rabs 4, 5, 7, 8, and 9 were mainly localised within a peri-nuclear compartment facing away from the furrow. Using RNA interference and dominant negative Rab mutants, we evaluated the role of these Rabs in furrowing and abscission. Consistent with previous work, we find that Rab 11 is intimately involved in abscission. However, we further found that depletion of Rab 4 slowed but did not prevent abscission. Depletion of any other Rab species had little effect on furrowing or abscission. These data suggest that the membrane trafficking events required for completion of cytokinesis are largely controlled by Rab 11 and not other endosomal Rab proteins, and further suggest that the relocation of Rab 11-specific cargo is an integral facet of abscission. Arf6 knockdown was without effect on cytokinesis, but when both Rab 11 and Arf6 were knocked-down, we found the furrow rapidly regressed and the cells were unable to form a stable midbody. We suggest that Rab 11 and Arf6 function synergistically in the switch from furrowing to abscission, as well as in the terminal stage of abscission.
LanguageEnglish
Pages25-35
Number of pages11
JournalEuropean Journal of Cell Biology
Volume86
Issue number1
Early online date8 Dec 2006
DOIs
Publication statusPublished - 17 Jan 2007

Fingerprint

Cytokinesis
GTP Phosphohydrolases
Membranes
Telophase
RNA Interference
Proteins

Keywords

  • cytokenesis
  • abscission
  • Rab GTPases

Cite this

Yu, Xinzi ; Prekeris, Rytis ; Gould, Gwyn W. / Role of endosomal Fab GTPases in cytokinesis. In: European Journal of Cell Biology . 2007 ; Vol. 86, No. 1. pp. 25-35.
@article{e01ba64a964549e6b93d4b74249d0936,
title = "Role of endosomal Fab GTPases in cytokinesis",
abstract = "Completion of cytokinesis requires Rab 11-dependent membrane trafficking events to deliver new membrane to the furrow and for abscission. Many Rabs have overlapping endosomal distributions, hence, we examined whether these Rabs also function in cytokinesis. Analysis of the distribution of Rabs 4, 5, 7, 8, 9, 11, 21, and 22 revealed that only Rab 11 was enriched within the furrow of cells in telophase or present within the midbody. By contrast, Rabs 4, 5, 7, 8, and 9 were mainly localised within a peri-nuclear compartment facing away from the furrow. Using RNA interference and dominant negative Rab mutants, we evaluated the role of these Rabs in furrowing and abscission. Consistent with previous work, we find that Rab 11 is intimately involved in abscission. However, we further found that depletion of Rab 4 slowed but did not prevent abscission. Depletion of any other Rab species had little effect on furrowing or abscission. These data suggest that the membrane trafficking events required for completion of cytokinesis are largely controlled by Rab 11 and not other endosomal Rab proteins, and further suggest that the relocation of Rab 11-specific cargo is an integral facet of abscission. Arf6 knockdown was without effect on cytokinesis, but when both Rab 11 and Arf6 were knocked-down, we found the furrow rapidly regressed and the cells were unable to form a stable midbody. We suggest that Rab 11 and Arf6 function synergistically in the switch from furrowing to abscission, as well as in the terminal stage of abscission.",
keywords = "cytokenesis, abscission, Rab GTPases",
author = "Xinzi Yu and Rytis Prekeris and Gould, {Gwyn W.}",
year = "2007",
month = "1",
day = "17",
doi = "10.1016/j.ejcb.2006.10.002",
language = "English",
volume = "86",
pages = "25--35",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
number = "1",

}

Role of endosomal Fab GTPases in cytokinesis. / Yu, Xinzi; Prekeris, Rytis; Gould, Gwyn W.

In: European Journal of Cell Biology , Vol. 86, No. 1, 17.01.2007, p. 25-35.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Role of endosomal Fab GTPases in cytokinesis

AU - Yu, Xinzi

AU - Prekeris, Rytis

AU - Gould, Gwyn W.

PY - 2007/1/17

Y1 - 2007/1/17

N2 - Completion of cytokinesis requires Rab 11-dependent membrane trafficking events to deliver new membrane to the furrow and for abscission. Many Rabs have overlapping endosomal distributions, hence, we examined whether these Rabs also function in cytokinesis. Analysis of the distribution of Rabs 4, 5, 7, 8, 9, 11, 21, and 22 revealed that only Rab 11 was enriched within the furrow of cells in telophase or present within the midbody. By contrast, Rabs 4, 5, 7, 8, and 9 were mainly localised within a peri-nuclear compartment facing away from the furrow. Using RNA interference and dominant negative Rab mutants, we evaluated the role of these Rabs in furrowing and abscission. Consistent with previous work, we find that Rab 11 is intimately involved in abscission. However, we further found that depletion of Rab 4 slowed but did not prevent abscission. Depletion of any other Rab species had little effect on furrowing or abscission. These data suggest that the membrane trafficking events required for completion of cytokinesis are largely controlled by Rab 11 and not other endosomal Rab proteins, and further suggest that the relocation of Rab 11-specific cargo is an integral facet of abscission. Arf6 knockdown was without effect on cytokinesis, but when both Rab 11 and Arf6 were knocked-down, we found the furrow rapidly regressed and the cells were unable to form a stable midbody. We suggest that Rab 11 and Arf6 function synergistically in the switch from furrowing to abscission, as well as in the terminal stage of abscission.

AB - Completion of cytokinesis requires Rab 11-dependent membrane trafficking events to deliver new membrane to the furrow and for abscission. Many Rabs have overlapping endosomal distributions, hence, we examined whether these Rabs also function in cytokinesis. Analysis of the distribution of Rabs 4, 5, 7, 8, 9, 11, 21, and 22 revealed that only Rab 11 was enriched within the furrow of cells in telophase or present within the midbody. By contrast, Rabs 4, 5, 7, 8, and 9 were mainly localised within a peri-nuclear compartment facing away from the furrow. Using RNA interference and dominant negative Rab mutants, we evaluated the role of these Rabs in furrowing and abscission. Consistent with previous work, we find that Rab 11 is intimately involved in abscission. However, we further found that depletion of Rab 4 slowed but did not prevent abscission. Depletion of any other Rab species had little effect on furrowing or abscission. These data suggest that the membrane trafficking events required for completion of cytokinesis are largely controlled by Rab 11 and not other endosomal Rab proteins, and further suggest that the relocation of Rab 11-specific cargo is an integral facet of abscission. Arf6 knockdown was without effect on cytokinesis, but when both Rab 11 and Arf6 were knocked-down, we found the furrow rapidly regressed and the cells were unable to form a stable midbody. We suggest that Rab 11 and Arf6 function synergistically in the switch from furrowing to abscission, as well as in the terminal stage of abscission.

KW - cytokenesis

KW - abscission

KW - Rab GTPases

U2 - 10.1016/j.ejcb.2006.10.002

DO - 10.1016/j.ejcb.2006.10.002

M3 - Article

VL - 86

SP - 25

EP - 35

JO - European Journal of Cell Biology

T2 - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 1

ER -