Role of amyloid-beta glycine 33 in oligomerization, toxicity, and neuronal plasticity

Anja Harmeier, Christian Wozny, Benjamin R Rost, Lisa-Marie Munter, Haiqing Hua, Oleg Georgiev, Michael Beyermann, Peter W. Hildebrand, Christoph Weise, Walter Schaffner, Dietmar Schmitz, Gerd Multhaup

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The aggregation of the amyloid-beta (Abeta) peptide plays a pivotal role in the pathogenesis of Alzheimer's disease, as soluble oligomers are intimately linked to neuronal toxicity and inhibition of hippocampal long-term potentiation (LTP). In the C-terminal region of Abeta there are three consecutive GxxxG dimerization motifs, which we could previously demonstrate to play a critical role in the generation of Abeta. Here, we show that glycine 33 (G33) of the central GxxxG interaction motif within the hydrophobic Abeta sequence is important for the aggregation dynamics of the peptide. Abeta peptides with alanine or isoleucine substitutions of G33 displayed an increased propensity to form higher oligomers, which we could attribute to conformational changes. Importantly, the oligomers of G33 variants were much less toxic than Abeta(42) wild type (WT), in vitro and in vivo. Also, whereas Abeta(42) WT is known to inhibit LTP, Abeta(42) G33 variants had lost the potential to inhibit LTP. Our findings reveal that conformational changes induced by G33 substitutions unlink toxicity and oligomerization of Abeta on the molecular level and suggest that G33 is the key amino acid in the toxic activity of Abeta. Thus, a specific toxic conformation of Abeta exists, which represents a promising target for therapeutic interventions.

Original languageEnglish
Pages (from-to)7582-7590
Number of pages9
JournalJournal of Neuroscience
Volume29
Issue number23
DOIs
Publication statusPublished - 10 Jun 2009

Keywords

  • amino acid motifs
  • amino acid sequence
  • amino acid substitution
  • amyloid beta-peptides
  • animals
  • cell death
  • cell line, tumor
  • cells, cultured
  • Drosophila melanogaster
  • excitatory postsynaptic potentials
  • eye
  • glycine
  • hippocampus
  • humans
  • hydrophobic and hydrophilic interactions
  • long-term potentiation
  • models, molecular
  • molecular sequence data
  • mutation, missense
  • neuronal plasticity
  • neurons
  • peptide fragments
  • protein conformation
  • protein multimerization
  • rats
  • rats, wistar

Fingerprint Dive into the research topics of 'Role of amyloid-beta glycine 33 in oligomerization, toxicity, and neuronal plasticity'. Together they form a unique fingerprint.

  • Cite this

    Harmeier, A., Wozny, C., Rost, B. R., Munter, L-M., Hua, H., Georgiev, O., ... Multhaup, G. (2009). Role of amyloid-beta glycine 33 in oligomerization, toxicity, and neuronal plasticity. Journal of Neuroscience, 29(23), 7582-7590. https://doi.org/10.1523/JNEUROSCI.1336-09.2009