RNA stability in frozen/thawed clinical blood samples

Olga Maria Posada Estefan, Rothwelle Tate, Mary Grant

Research output: Contribution to conferenceAbstract

Abstract

The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial
we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.
LanguageEnglish
Pages9
Number of pages1
Publication statusPublished - 2011
EventBritish Toxicology Society Meeting - Nottingham, United Kingdom
Duration: 5 Sep 20116 Sep 2011

Conference

ConferenceBritish Toxicology Society Meeting
CountryUnited Kingdom
CityNottingham
Period5/09/116/09/11

Fingerprint

RNA Stability
RNA
Gene Expression

Keywords

  • genes
  • blood cells
  • RNA stability
  • gene expression analysis

Cite this

Posada Estefan, O. M., Tate, R., & Grant, M. (2011). RNA stability in frozen/thawed clinical blood samples. 9. Abstract from British Toxicology Society Meeting, Nottingham, United Kingdom.
Posada Estefan, Olga Maria ; Tate, Rothwelle ; Grant, Mary. / RNA stability in frozen/thawed clinical blood samples. Abstract from British Toxicology Society Meeting, Nottingham, United Kingdom.1 p.
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author = "{Posada Estefan}, {Olga Maria} and Rothwelle Tate and Mary Grant",
year = "2011",
language = "English",
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Posada Estefan, OM, Tate, R & Grant, M 2011, 'RNA stability in frozen/thawed clinical blood samples' British Toxicology Society Meeting, Nottingham, United Kingdom, 5/09/11 - 6/09/11, pp. 9.

RNA stability in frozen/thawed clinical blood samples. / Posada Estefan, Olga Maria; Tate, Rothwelle; Grant, Mary.

2011. 9 Abstract from British Toxicology Society Meeting, Nottingham, United Kingdom.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - RNA stability in frozen/thawed clinical blood samples

AU - Posada Estefan, Olga Maria

AU - Tate, Rothwelle

AU - Grant, Mary

PY - 2011

Y1 - 2011

N2 - The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.

AB - The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.

KW - genes

KW - blood cells

KW - RNA stability

KW - gene expression analysis

UR - http://www.robots.ox.ac.uk/~davidc/pubs/pgbiomed_proc2011.pdf

M3 - Abstract

SP - 9

ER -

Posada Estefan OM, Tate R, Grant M. RNA stability in frozen/thawed clinical blood samples. 2011. Abstract from British Toxicology Society Meeting, Nottingham, United Kingdom.