RNA stability in frozen/thawed clinical blood samples

Olga Maria Posada Estefan, Rothwelle Tate, Mary Grant

Research output: Contribution to conferenceAbstract

Abstract

The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial
we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.
Original languageEnglish
Pages9
Number of pages1
Publication statusPublished - 2011
EventBritish Toxicology Society Meeting - Nottingham, United Kingdom
Duration: 5 Sep 20116 Sep 2011

Conference

ConferenceBritish Toxicology Society Meeting
CountryUnited Kingdom
CityNottingham
Period5/09/116/09/11

Keywords

  • genes
  • blood cells
  • RNA stability
  • gene expression analysis

Cite this

Posada Estefan, O. M., Tate, R., & Grant, M. (2011). RNA stability in frozen/thawed clinical blood samples. 9. Abstract from British Toxicology Society Meeting, Nottingham, United Kingdom.