Risk stratification of cervical disease using detection of human papillomavirus (HPV) E4 protein and cellular MCM protein in clinical liquid based cytology samples

Andrew Stevenson, Kim Kavanagh, Jiafeng Pan, Lynne Stevenson, Heather Griffin, John Doorbar, Evelyn Scott, Miriam Deeny, Kate Cuschieri, Sheila V. Graham

Research output: Contribution to journalArticle

Abstract

Background: While human papillomavirus (HPV) DNA testing offers high sensitivity for the detection of significant cervical disease, its specificity is suboptimal given the high prevalence of transient HPV infections (CIN1 or less). Biomarkers to identify those suffering from low grade disease from those with high grade disease could save healthcare costs and reduce patient anxiety. Objective: The objective of the present work was to develop and test an immunohistochemistry (IHC)-based dual viral and cellular biomarker strategy which was applicable to liquid based cytology (LBC) samples. Study design: We developed a novel IHC assay for detection of HPV E4 and cellular minichromosome maintenance (MCM) proteins in routinely taken cervical LBC samples using cytospin-prepared slides. The assay was applied to a prospective cohort of Scottish women referred to a colposcopy clinic due to preceding cytological abnormalities. The performance of the biomarkers for detection of clinically insignificant (CIN1 or less) versus significant disease was determined. Results: A total of 81 women were recruited representing 64 cases of<=CIN1 and 28 of CIN2+. Biomarker performance relative to histopathology outcomes showed high levels of MCM detection was significantly associated with CIN2+ (p=0.03) while E4 was detected more frequently in<=CIN1 (p=0.06). Conclusions: Combined detection of a host proliferation marker and a marker of viral gene expression could allow triage of cases of clinically insignificant disease prior to colposcopy. However, there was overlap between distributions of MCM levels in CIN2+ and<=CIN1 suggesting that additional biomarkers would be required for improved specificity. Combined with cytospin-prepared slides this approach could provide a means of risk stratification of disease in low resource settings.
LanguageEnglish
Pages19-25
Number of pages7
JournalJournal of Clinical Virology
Volume108
Early online date31 Aug 2018
DOIs
Publication statusPublished - 30 Nov 2018

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Minichromosome Maintenance Proteins
Cell Biology
Biomarkers
Colposcopy
Proteins
Immunohistochemistry
Maintenance
Papillomavirus Infections
Viral Genes
Triage
Psychological Stress
Health Care Costs
Anxiety
Gene Expression
DNA

Keywords

  • HPV
  • cervical intraepithelial neoplasia
  • biomarkers
  • liquid based cytology
  • cytospin

Cite this

Stevenson, Andrew ; Kavanagh, Kim ; Pan, Jiafeng ; Stevenson, Lynne ; Griffin, Heather ; Doorbar, John ; Scott, Evelyn ; Deeny, Miriam ; Cuschieri, Kate ; Graham, Sheila V. / Risk stratification of cervical disease using detection of human papillomavirus (HPV) E4 protein and cellular MCM protein in clinical liquid based cytology samples. In: Journal of Clinical Virology. 2018 ; Vol. 108. pp. 19-25.
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Risk stratification of cervical disease using detection of human papillomavirus (HPV) E4 protein and cellular MCM protein in clinical liquid based cytology samples. / Stevenson, Andrew; Kavanagh, Kim; Pan, Jiafeng; Stevenson, Lynne; Griffin, Heather; Doorbar, John ; Scott, Evelyn ; Deeny, Miriam ; Cuschieri, Kate; Graham, Sheila V.

In: Journal of Clinical Virology, Vol. 108, 30.11.2018, p. 19-25.

Research output: Contribution to journalArticle

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AU - Stevenson, Andrew

AU - Kavanagh, Kim

AU - Pan, Jiafeng

AU - Stevenson, Lynne

AU - Griffin, Heather

AU - Doorbar, John

AU - Scott, Evelyn

AU - Deeny, Miriam

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AU - Graham, Sheila V.

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