Revolutionizing prokaryotic systematics through next-generation sequencing

Vartul Sangal; Leena Nieminen; Nicholas P. Tucker; Paul A. Hoskisson

doi:10.1016/bs.mim.2014.07.001

Revolutionizing prokaryotic systematics through next-generation sequencing

Vartul Sangal, Leena Nieminen, Nicholas P. Tucker, Paul A. Hoskisson^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

10 Citations (Scopus)

Abstract

Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.

Original language	English
Title of host publication	New Approaches to Prokaryotic Systematics
Editors	Michael Goodfellow, Iain Sutcliffe, Jongsik Chun
Pages	75-101
Number of pages	27
DOIs	https://doi.org/10.1016/bs.mim.2014.07.001
Publication status	Published - 28 Aug 2014

Publication series

Name	Methods in Microbiology
Publisher	Elsevier
Volume	41

Keywords

bioinformatic tools
comparative genomics
de novo assembly
genome annotation
mapping
next-generation sequencing
phylogenetic analyses
prokaryotic systematics

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10.1016/bs.mim.2014.07.001

Cite this

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title = "Revolutionizing prokaryotic systematics through next-generation sequencing",

abstract = "Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.",

keywords = "bioinformatic tools, comparative genomics, de novo assembly, genome annotation, mapping, next-generation sequencing, phylogenetic analyses, prokaryotic systematics",

author = "Vartul Sangal and Leena Nieminen and Tucker, {Nicholas P.} and Hoskisson, {Paul A.}",

year = "2014",

month = aug,

day = "28",

doi = "10.1016/bs.mim.2014.07.001",

language = "English",

isbn = "978-0-12-800176-9 ",

series = "Methods in Microbiology",

publisher = "Elsevier",

pages = "75--101",

editor = "Michael Goodfellow and Iain Sutcliffe and Jongsik Chun",

booktitle = "New Approaches to Prokaryotic Systematics",

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TY - CHAP

T1 - Revolutionizing prokaryotic systematics through next-generation sequencing

AU - Sangal, Vartul

AU - Nieminen, Leena

AU - Tucker, Nicholas P.

AU - Hoskisson, Paul A.

PY - 2014/8/28

Y1 - 2014/8/28

N2 - Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.

AB - Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.

KW - bioinformatic tools

KW - comparative genomics

KW - de novo assembly

KW - genome annotation

KW - mapping

KW - next-generation sequencing

KW - phylogenetic analyses

KW - prokaryotic systematics

UR - http://www.scopus.com/inward/record.url?scp=84919419857&partnerID=8YFLogxK

U2 - 10.1016/bs.mim.2014.07.001

DO - 10.1016/bs.mim.2014.07.001

M3 - Chapter

AN - SCOPUS:84919419857

SN - 978-0-12-800176-9

T3 - Methods in Microbiology

SP - 75

EP - 101

BT - New Approaches to Prokaryotic Systematics

A2 - Goodfellow, Michael

A2 - Sutcliffe, Iain

A2 - Chun, Jongsik

ER -

Revolutionizing prokaryotic systematics through next-generation sequencing

Abstract

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Comparative genomics of Pseudomonas aeruginosa

Comparative genomics of Pseudomonas aeruginosa

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Revolutionizing prokaryotic systematics through next-generation sequencing

Abstract

Publication series

Keywords

Access to Document

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Comparative genomics of Pseudomonas aeruginosa

Comparative genomics of Pseudomonas aeruginosa

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