Abstract
Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.
| Language | English |
|---|---|
| Title of host publication | New Approaches to Prokaryotic Systematics |
| Editors | Michael Goodfellow, Iain Sutcliffe, Jongsik Chun |
| Pages | 75-101 |
| Number of pages | 27 |
| DOIs | |
| Publication status | Published - 28 Aug 2014 |
Publication series
| Name | Methods in Microbiology |
|---|---|
| Publisher | Elsevier |
| Volume | 41 |
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Keywords
- bioinformatic tools
- comparative genomics
- de novo assembly
- genome annotation
- mapping
- next-generation sequencing
- phylogenetic analyses
- prokaryotic systematics
Cite this
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Revolutionizing prokaryotic systematics through next-generation sequencing. / Sangal, Vartul; Nieminen, Leena; Tucker, Nicholas P.; Hoskisson, Paul A.
New Approaches to Prokaryotic Systematics. ed. / Michael Goodfellow; Iain Sutcliffe; Jongsik Chun. 2014. p. 75-101 (Methods in Microbiology; Vol. 41).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Revolutionizing prokaryotic systematics through next-generation sequencing
AU - Sangal, Vartul
AU - Nieminen, Leena
AU - Tucker, Nicholas P.
AU - Hoskisson, Paul A.
PY - 2014/8/28
Y1 - 2014/8/28
N2 - Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.
AB - Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.
KW - bioinformatic tools
KW - comparative genomics
KW - de novo assembly
KW - genome annotation
KW - mapping
KW - next-generation sequencing
KW - phylogenetic analyses
KW - prokaryotic systematics
UR - http://www.scopus.com/inward/record.url?scp=84919419857&partnerID=8YFLogxK
U2 - 10.1016/bs.mim.2014.07.001
DO - 10.1016/bs.mim.2014.07.001
M3 - Chapter
SN - 978-0-12-800176-9
T3 - Methods in Microbiology
SP - 75
EP - 101
BT - New Approaches to Prokaryotic Systematics
A2 - Goodfellow, Michael
A2 - Sutcliffe, Iain
A2 - Chun, Jongsik
ER -