Revolutionizing prokaryotic systematics through next-generation sequencing

Vartul Sangal; Leena Nieminen; Nicholas P. Tucker; Paul A. Hoskisson

doi:10.1016/bs.mim.2014.07.001

Revolutionizing prokaryotic systematics through next-generation sequencing

Vartul Sangal, Leena Nieminen, Nicholas P. Tucker, Paul A. Hoskisson

Research output: Chapter in Book/Report/Conference proceeding › Chapter

8 Citations (Scopus)

Abstract

Next-generation sequencing has revolutionized modern research in all life sciences. It has been applied in studying evolution, global transmission and local adaptation of pathogens, microbial ecology, genetics and physiology. In this chapter, we have provided a brief summary of recent advances in sequencing technologies and easy to use bioinformatic tools to analyse sequence data, with a focus on prokaryotic biology. We have emphasized the importance of routinely applying whole genome sequencing in prokaryotic systematics, evolution, genotyping and functional characterization.

Original language	English
Title of host publication	New Approaches to Prokaryotic Systematics
Editors	Michael Goodfellow, Iain Sutcliffe, Jongsik Chun
Pages	75-101
Number of pages	27
DOIs	https://doi.org/10.1016/bs.mim.2014.07.001
Publication status	Published - 28 Aug 2014

Publication series

Name	Methods in Microbiology
Publisher	Elsevier
Volume	41

Keywords

bioinformatic tools
comparative genomics
de novo assembly
genome annotation
mapping
next-generation sequencing
phylogenetic analyses
prokaryotic systematics

Access to Document

10.1016/bs.mim.2014.07.001

Link to publication in Scopus

Activities

2 Invited talk

Comparative genomics of Pseudomonas aeruginosa

Nicholas Tucker (Contributor)

Oct 2013

Activity: Talk or presentation types › Invited talk

Comparative genomics of Pseudomonas aeruginosa

Nicholas Tucker (Contributor)

25 Jun 2012

Activity: Talk or presentation types › Invited talk

Cite this

Sangal, V., Nieminen, L., Tucker, N. P., & Hoskisson, P. A. (2014). Revolutionizing prokaryotic systematics through next-generation sequencing. In M. Goodfellow, I. Sutcliffe, & J. Chun (Eds.), New Approaches to Prokaryotic Systematics (pp. 75-101). (Methods in Microbiology; Vol. 41). https://doi.org/10.1016/bs.mim.2014.07.001

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Revolutionizing prokaryotic systematics through next-generation sequencing. / Sangal, Vartul; Nieminen, Leena; Tucker, Nicholas P.; Hoskisson, Paul A.

New Approaches to Prokaryotic Systematics. ed. / Michael Goodfellow; Iain Sutcliffe; Jongsik Chun. 2014. p. 75-101 (Methods in Microbiology; Vol. 41).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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