Reversible DNA micro-patterning using the fluorous effect

Gabriella Flynn; Jamie Withers; Gerard Macias; Justin R. Sperlin; Sarah L. Henry; Jonathan M. Cooper; Glenn A. Burley; Alasdair W. Clark

doi:10.1039/C7CC00288B

Reversible DNA micro-patterning using the fluorous effect

Gabriella Flynn, Jamie Withers, Gerard Macias, Justin R. Sperlin, Sarah L. Henry, Jonathan M. Cooper, Glenn A. Burley, Alasdair W. Clark

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

104 Downloads (Pure)

Abstract

We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.

Original language	English
Pages (from-to)	3094-3097
Number of pages	4
Journal	Chemical Communications
Volume	53
Issue number	21
DOIs	https://doi.org/10.1039/C7CC00288B
Publication status	Published - 23 Feb 2017

Keywords

DNA immobilisation
sub-micron resolution
fluorous effect
molecular surface patterning
oligodeoxyribonucleotides
nonspecific binding

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10.1039/C7CC00288BLicence: CC BY 3.0

Flynn-etal-CC-2017-Reversible-DNA-micro-patterning-using-the-fluorous-effectFinal published version, 2.05 MBLicence: CC BY 3.0

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2 Finished

Transformable photonic circuitry: using DNA to create the next generation of molecularly active surfaces
Burley, G.
Leverhulme Trust
22/06/15 → 21/12/17
Project: Research
Transformable photonic circuitry: using DNA to create the next generation of molecularly active surfaces
Withers, J. & Burley, G.
22/06/15 → 21/12/17
Project: Research

Cite this

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abstract = "We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.",

keywords = "DNA immobilisation, sub-micron resolution, fluorous effect, molecular surface patterning, oligodeoxyribonucleotides, nonspecific binding",

author = "Gabriella Flynn and Jamie Withers and Gerard Macias and Sperlin, {Justin R.} and Henry, {Sarah L.} and Cooper, {Jonathan M.} and Burley, {Glenn A.} and Clark, {Alasdair W.}",

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AU - Flynn, Gabriella

AU - Withers, Jamie

AU - Macias, Gerard

AU - Sperlin, Justin R.

AU - Henry, Sarah L.

AU - Cooper, Jonathan M.

AU - Burley, Glenn A.

AU - Clark, Alasdair W.

PY - 2017/2/23

Y1 - 2017/2/23

N2 - We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.

AB - We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.

KW - DNA immobilisation

KW - sub-micron resolution

KW - fluorous effect

KW - molecular surface patterning

KW - oligodeoxyribonucleotides

KW - nonspecific binding

UR - http://dx.doi.org/ 10.5525/gla.researchdata.380

U2 - 10.1039/C7CC00288B

DO - 10.1039/C7CC00288B

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EP - 3097

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

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ER -

Reversible DNA micro-patterning using the fluorous effect

Abstract

Keywords

Access to Document

Transformable photonic circuitry: using DNA to create the next generation of molecularly active surfaces

Transformable photonic circuitry: using DNA to create the next generation of molecularly active surfaces

Cite this