Reversible DNA micro-patterning using the fluorous effect

Gabriella Flynn, Jamie Withers, Gerard Macias, Justin R. Sperlin, Sarah L. Henry, Jonathan M. Cooper, Glenn A. Burley, Alasdair W. Clark

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.
LanguageEnglish
Pages3094-3097
Number of pages4
JournalChemical Communications
Volume53
Issue number21
DOIs
Publication statusPublished - 23 Feb 2017

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DNA sequences
DNA
Degradation

Keywords

  • DNA immobilisation
  • sub-micron resolution
  • fluorous effect
  • molecular surface patterning
  • oligodeoxyribonucleotides
  • nonspecific binding

Cite this

Flynn, G., Withers, J., Macias, G., Sperlin, J. R., Henry, S. L., Cooper, J. M., ... Clark, A. W. (2017). Reversible DNA micro-patterning using the fluorous effect. Chemical Communications, 53(21), 3094-3097. https://doi.org/10.1039/C7CC00288B
Flynn, Gabriella ; Withers, Jamie ; Macias, Gerard ; Sperlin, Justin R. ; Henry, Sarah L. ; Cooper, Jonathan M. ; Burley, Glenn A. ; Clark, Alasdair W. / Reversible DNA micro-patterning using the fluorous effect. In: Chemical Communications. 2017 ; Vol. 53, No. 21. pp. 3094-3097.
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Flynn, G, Withers, J, Macias, G, Sperlin, JR, Henry, SL, Cooper, JM, Burley, GA & Clark, AW 2017, 'Reversible DNA micro-patterning using the fluorous effect' Chemical Communications, vol. 53, no. 21, pp. 3094-3097. https://doi.org/10.1039/C7CC00288B

Reversible DNA micro-patterning using the fluorous effect. / Flynn, Gabriella; Withers, Jamie; Macias, Gerard; Sperlin, Justin R.; Henry, Sarah L.; Cooper, Jonathan M.; Burley, Glenn A.; Clark, Alasdair W.

In: Chemical Communications, Vol. 53, No. 21, 23.02.2017, p. 3094-3097.

Research output: Contribution to journalArticle

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AU - Flynn, Gabriella

AU - Withers, Jamie

AU - Macias, Gerard

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AU - Burley, Glenn A.

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KW - DNA immobilisation

KW - sub-micron resolution

KW - fluorous effect

KW - molecular surface patterning

KW - oligodeoxyribonucleotides

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Flynn G, Withers J, Macias G, Sperlin JR, Henry SL, Cooper JM et al. Reversible DNA micro-patterning using the fluorous effect. Chemical Communications. 2017 Feb 23;53(21):3094-3097. https://doi.org/10.1039/C7CC00288B