Reversibility of neuromuscular blockade produced by toxins isolated from the venom of the seasnake Laticauda semifasciata

A. L. Harvey, I. W. Rodger

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The neuromuscular blocking potencies and reversibilities of purified toxins from the venom of the seasnake Laticauda semifasciata were assessed on the chick biventer cervicis nerve-muscle preparation. All toxins inhibited the responses to indirect stimulation and to carbachol but not the responses to direct muscle stimulation or to raised KCl concentrations. Fraction 0·06 M and 0·09 M were about 100 times less potent than the most potent compound, erabutoxin b but they were similarly partially and slowly reversible. In contrast LsIII toxin was less potent than erabutoxin b but its effect could be readily reversed by washing. Since the toxins differ in chemical structure, it is suggested that separate parts of the toxin molecule may determine affinity for the acetylcholine receptor and reversibility.

LanguageEnglish
Pages219-225
Number of pages7
JournalToxicon
Volume16
Issue number3
DOIs
Publication statusPublished - 1 Jan 1978

Fingerprint

Neuromuscular Blockade
Venoms
Muscle
Neuromuscular Junction
Carbachol
Cholinergic Receptors
Washing
Muscles
Molecules
erabutoxin b

Keywords

  • carbachol
  • cholinergic receptor
  • erabutoxin
  • snake venom
  • animal experiment
  • chicken
  • muscle
  • neuromuscular blocking
  • peripheral nervous system
  • reptile

Cite this

@article{6616ec0bec024ad581c14aa35993f426,
title = "Reversibility of neuromuscular blockade produced by toxins isolated from the venom of the seasnake Laticauda semifasciata",
abstract = "The neuromuscular blocking potencies and reversibilities of purified toxins from the venom of the seasnake Laticauda semifasciata were assessed on the chick biventer cervicis nerve-muscle preparation. All toxins inhibited the responses to indirect stimulation and to carbachol but not the responses to direct muscle stimulation or to raised KCl concentrations. Fraction 0·06 M and 0·09 M were about 100 times less potent than the most potent compound, erabutoxin b but they were similarly partially and slowly reversible. In contrast LsIII toxin was less potent than erabutoxin b but its effect could be readily reversed by washing. Since the toxins differ in chemical structure, it is suggested that separate parts of the toxin molecule may determine affinity for the acetylcholine receptor and reversibility.",
keywords = "carbachol, cholinergic receptor, erabutoxin, snake venom, animal experiment, chicken, muscle, neuromuscular blocking, peripheral nervous system, reptile",
author = "Harvey, {A. L.} and Rodger, {I. W.}",
year = "1978",
month = "1",
day = "1",
doi = "10.1016/0041-0101(78)90082-X",
language = "English",
volume = "16",
pages = "219--225",
journal = "Toxicon",
issn = "0041-0101",
number = "3",

}

Reversibility of neuromuscular blockade produced by toxins isolated from the venom of the seasnake Laticauda semifasciata. / Harvey, A. L.; Rodger, I. W.

In: Toxicon, Vol. 16, No. 3, 01.01.1978, p. 219-225.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reversibility of neuromuscular blockade produced by toxins isolated from the venom of the seasnake Laticauda semifasciata

AU - Harvey, A. L.

AU - Rodger, I. W.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - The neuromuscular blocking potencies and reversibilities of purified toxins from the venom of the seasnake Laticauda semifasciata were assessed on the chick biventer cervicis nerve-muscle preparation. All toxins inhibited the responses to indirect stimulation and to carbachol but not the responses to direct muscle stimulation or to raised KCl concentrations. Fraction 0·06 M and 0·09 M were about 100 times less potent than the most potent compound, erabutoxin b but they were similarly partially and slowly reversible. In contrast LsIII toxin was less potent than erabutoxin b but its effect could be readily reversed by washing. Since the toxins differ in chemical structure, it is suggested that separate parts of the toxin molecule may determine affinity for the acetylcholine receptor and reversibility.

AB - The neuromuscular blocking potencies and reversibilities of purified toxins from the venom of the seasnake Laticauda semifasciata were assessed on the chick biventer cervicis nerve-muscle preparation. All toxins inhibited the responses to indirect stimulation and to carbachol but not the responses to direct muscle stimulation or to raised KCl concentrations. Fraction 0·06 M and 0·09 M were about 100 times less potent than the most potent compound, erabutoxin b but they were similarly partially and slowly reversible. In contrast LsIII toxin was less potent than erabutoxin b but its effect could be readily reversed by washing. Since the toxins differ in chemical structure, it is suggested that separate parts of the toxin molecule may determine affinity for the acetylcholine receptor and reversibility.

KW - carbachol

KW - cholinergic receptor

KW - erabutoxin

KW - snake venom

KW - animal experiment

KW - chicken

KW - muscle

KW - neuromuscular blocking

KW - peripheral nervous system

KW - reptile

UR - http://www.scopus.com/inward/record.url?scp=0018096654&partnerID=8YFLogxK

U2 - 10.1016/0041-0101(78)90082-X

DO - 10.1016/0041-0101(78)90082-X

M3 - Article

VL - 16

SP - 219

EP - 225

JO - Toxicon

T2 - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 3

ER -