Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke Wei, Dorin Simionesie, Jan Sefcik, Jens U. Sutter, Qingjiang Xue, Jun Yu, Jinliang Wang, David J.S. Birch, Yu Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.
LanguageEnglish
Pages5738–5741
Number of pages4
JournalOptics Letters
Volume40
Issue number24
DOIs
Publication statusPublished - 9 Dec 2015

Fingerprint

Fluorescence Spectrometry
Gold
gold
fluorescence
spectroscopy
Fluorescence
Energy Transfer
Photons
stems
electrodynamics
Nanoparticles
spacers
counting
deoxyribonucleic acid
energy transfer
quenching
nanoparticles
DNA
kinetics
photons

Keywords

  • Au-nanobeacons
  • gold nanobeacons
  • fluorescence

Cite this

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title = "Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy",
abstract = "We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60{\%} increase in decay time component and 300{\%} increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.",
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Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy. / Wei, Guoke ; Simionesie, Dorin; Sefcik, Jan; Sutter, Jens U.; Xue, Qingjiang; Yu, Jun; Wang, Jinliang; Birch, David J.S.; Chen, Yu.

In: Optics Letters, Vol. 40, No. 24, 09.12.2015, p. 5738–5741 .

Research output: Contribution to journalArticle

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T1 - Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

AU - Wei, Guoke

AU - Simionesie, Dorin

AU - Sefcik, Jan

AU - Sutter, Jens U.

AU - Xue, Qingjiang

AU - Yu, Jun

AU - Wang, Jinliang

AU - Birch, David J.S.

AU - Chen, Yu

N1 - © 2015 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.

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Y1 - 2015/12/9

N2 - We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

AB - We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

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