Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke  Wei; Dorin Simionesie; Jan Sefcik; Jens U. Sutter; Qingjiang Xue; Jun Yu; Jinliang Wang; David J.S. Birch; Yu Chen

doi:10.1364/OL.40.005738

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke Wei, Dorin Simionesie, Jan Sefcik, Jens U. Sutter, Qingjiang Xue, Jun Yu, Jinliang Wang, David J.S. Birch, Yu Chen

Research output: Contribution to journal › Article

3 Citations (Scopus)

49 Downloads (Pure)

Abstract

We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

Original language	English
Pages (from-to)	5738–5741
Number of pages	4
Journal	Optics Letters
Volume	40
Issue number	24
DOIs	https://doi.org/10.1364/OL.40.005738
Publication status	Published - 9 Dec 2015

Keywords

Au-nanobeacons
gold nanobeacons
fluorescence

Access to Document

10.1364/OL.40.005738

Wei-etal-OL-2015-Revealing-the-photophysics-of-gold-nanobeacons-via-time-resolvedAccepted author manuscript, 1.19 MB

https://www.osapublishing.org/ol/abstract.cfm?uri=ol-40-24-5738

Projects

2 Finished

A new approach for imaging RNA at the single cell level

Chen, Y., Birch, D. & Yu, J.

BBSRC (Biotech & Biological Sciences Research Council)

29/07/13 → 28/01/15

Project: Research

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

Birch, D., Dawson, M., Faulds, K., Girkin, J., Graham, D., Martin, R., O'Donnell, K., Rolinski, O., Smith, W. & Wynne, K.

Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)

1/08/08 → 31/01/12

Project: Research

Research Output

3 Citations
1 Article

Fast bi-exponential fluorescence lifetime imaging analysis methods

Li, D. D-U., Yu, H. & Chen, Y., 1 Feb 2015, In : Optics Letters. 40, 3, p. 336-339 4 p.

Research output: Contribution to journal › Article

Open Access

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9 Citations (Scopus)

52 Downloads (Pure)

Cite this

Wei, G., Simionesie, D., Sefcik, J., Sutter, J. U., Xue, Q., Yu, J., Wang, J., Birch, D. J. S., & Chen, Y. (2015). Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy. Optics Letters, 40(24), 5738–5741 . https://doi.org/10.1364/OL.40.005738

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abstract = "We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons. ",

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