Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke Wei, Dorin Simionesie, Jan Sefcik, Jens U. Sutter, Qingjiang Xue, Jun Yu, Jinliang Wang, David J.S. Birch, Yu Chen

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Abstract

We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.
Original languageEnglish
Pages (from-to)5738–5741
Number of pages4
JournalOptics Letters
Volume40
Issue number24
DOIs
Publication statusPublished - 9 Dec 2015

Keywords

  • Au-nanobeacons
  • gold nanobeacons
  • fluorescence

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Research Output

  • 3 Citations
  • 1 Article

Fast bi-exponential fluorescence lifetime imaging analysis methods

Li, D. D-U., Yu, H. & Chen, Y., 1 Feb 2015, In : Optics Letters. 40, 3, p. 336-339 4 p.

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9 Citations (Scopus)
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