Abstract
| Language | English |
|---|---|
| Pages | 5738–5741 |
| Number of pages | 4 |
| Journal | Optics Letters |
| Volume | 40 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - 9 Dec 2015 |
Fingerprint
Keywords
- Au-nanobeacons
- gold nanobeacons
- fluorescence
Cite this
}
Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy. / Wei, Guoke ; Simionesie, Dorin; Sefcik, Jan; Sutter, Jens U.; Xue, Qingjiang; Yu, Jun; Wang, Jinliang; Birch, David J.S.; Chen, Yu.
In: Optics Letters, Vol. 40, No. 24, 09.12.2015, p. 5738–5741 .Research output: Contribution to journal › Article
TY - JOUR
T1 - Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy
AU - Wei, Guoke
AU - Simionesie, Dorin
AU - Sefcik, Jan
AU - Sutter, Jens U.
AU - Xue, Qingjiang
AU - Yu, Jun
AU - Wang, Jinliang
AU - Birch, David J.S.
AU - Chen, Yu
N1 - © 2015 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.
PY - 2015/12/9
Y1 - 2015/12/9
N2 - We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.
AB - We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.
KW - Au-nanobeacons
KW - gold nanobeacons
KW - fluorescence
UR - https://www.osapublishing.org/ol/abstract.cfm?uri=ol-40-24-5738
U2 - 10.1364/OL.40.005738
DO - 10.1364/OL.40.005738
M3 - Article
VL - 40
SP - 5738
EP - 5741
JO - Optics Letters
T2 - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 24
ER -