Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke  Wei; Dorin Simionesie; Jan Sefcik; Jens U. Sutter; Qingjiang Xue; Jun Yu; Jinliang Wang; David J.S. Birch; Yu Chen

doi:10.1364/OL.40.005738

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke Wei, Dorin Simionesie, Jan Sefcik, Jens U. Sutter, Qingjiang Xue, Jun Yu, Jinliang Wang, David J.S. Birch, Yu Chen

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Abstract

We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

Original language	English
Pages (from-to)	5738–5741
Number of pages	4
Journal	Optics Letters
Volume	40
Issue number	24
DOIs	https://doi.org/10.1364/OL.40.005738
Publication status	Published - 9 Dec 2015

Keywords

Au-nanobeacons
gold nanobeacons
fluorescence

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10.1364/OL.40.005738

Wei-etal-OL-2015-Revealing-the-photophysics-of-gold-nanobeacons-via-time-resolvedAccepted author manuscript, 1.19 MB

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title = "Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy",

abstract = "We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons. ",

keywords = "Au-nanobeacons, gold nanobeacons, fluorescence ",

author = "Guoke Wei and Dorin Simionesie and Jan Sefcik and Sutter, {Jens U.} and Qingjiang Xue and Jun Yu and Jinliang Wang and Birch, {David J.S.} and Yu Chen",

note = "{\textcopyright} 2015 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.",

year = "2015",

month = dec,

day = "9",

doi = "10.1364/OL.40.005738",

language = "English",

volume = "40",

pages = "5738–5741 ",

journal = "Optics Letters",

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T1 - Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

AU - Wei, Guoke

AU - Simionesie, Dorin

AU - Sefcik, Jan

AU - Sutter, Jens U.

AU - Xue, Qingjiang

AU - Yu, Jun

AU - Wang, Jinliang

AU - Birch, David J.S.

AU - Chen, Yu

N1 - © 2015 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.

PY - 2015/12/9

Y1 - 2015/12/9

N2 - We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

AB - We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modelling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

KW - Au-nanobeacons

KW - gold nanobeacons

KW - fluorescence

UR - https://www.osapublishing.org/ol/abstract.cfm?uri=ol-40-24-5738

U2 - 10.1364/OL.40.005738

DO - 10.1364/OL.40.005738

M3 - Article

SN - 0146-9592

VL - 40

SP - 5738

EP - 5741

JO - Optics Letters

JF - Optics Letters

IS - 24

ER -

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Abstract

Keywords

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A new approach for imaging RNA at the single cell level

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

Fast bi-exponential fluorescence lifetime imaging analysis methods

Cite this

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

A new approach for imaging RNA at the single cell level

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

Research output

Fast bi-exponential fluorescence lifetime imaging analysis methods

Cite this