Common carp (Cyprinus carpio) were treated in aquatic mesocosms with a single pulse of the herbicides atrazine or alachlor to study the bioavailability and biological activity of these herbicides using molecular indicators: Liver vitellogenin gene expression in male fish for estrogenic activity, liver cytochrome P4501A1 gene expression, and DNA damage in blood cells using the single-cell gel electrophoresis method. Both alachlor and atrazine showed dose-related increases in DNA strand breaks at environmentally relevant concentrations (<100 ppb). Gene expression indicators showed that neither herbicide had estrogenic activity in the carp, whereas atrazine at concentrations as low as 7 ppb induced cytochrome P4501A1 These results support the study of molecular indicators for exposure in surrogate ecosystems to gauge relevant environmental changes following herbicide treatments.
|Number of pages||7|
|Journal||Environmental Toxicology and Chemistry|
|Publication status||Published - Jan 2005|
- vitellogenin messenger ribonucleic acid
- fathead minnow
- nutrient levels
- 17 beta-estradiol
- reverse transcription-polymerase
- chain reaction
Chang, L. W., Toth, G. P., Gordon, D. A., Graham, D. W., Meier, J. R., Knapp, C. W., deNoyelles, F., Campbell, S., & Lattier, D. L. (2005). Responses of molecular indicators of exposure in mesocosms: common carp (cyprinus carpio) exposed to the herbicides alachlor and atrazine. Environmental Toxicology and Chemistry, 24(1), 190-197.