Resonance raman-spectroscopy of hemoglobin in intact-cells: A probe of oxygen-uptake by erythrocytes in rheumatoid arthritis

S. Hoey, D.H. Brown, A.A. McConnell, W.E. Smith, M. Marabani, R.D. Sturrock

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Resonance Raman spectra from intact viable erythrocytes can be used to study oxygen uptake in solution. In addition to changes in the oxidation state marker (v4), other bands due to the porphyrin ring (v3) and vinyl modes indicate subtle changes at oxygen pressures close to where the T/R change occurs. A comparison of whole cell and lysate spectra indicates a partial denaturation of hemoglobin on lysis. A simple smear technique is used to measure spectra from rheumatoid and normal blood. Results indicate a faster but less complete uptake of oxygen in cells from patients with rheumatoid arthritis than is the case in normal cell populations.
Original languageEnglish
Pages (from-to)189-199
Number of pages10
JournalJournal of Inorganic Biochemistry
Volume34
Issue number3
DOIs
Publication statusPublished - Nov 1988

Keywords

  • resonance raman-spectroscopy
  • hemoglobin
  • cells
  • oxygen-uptake
  • erythrocytes
  • rheumatoid arthritis

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