Resonance raman-spectroscopy of hemoglobin in intact-cells: A probe of oxygen-uptake by erythrocytes in rheumatoid arthritis

S. Hoey; D.H. Brown; A.A. McConnell; W.E. Smith; M. Marabani; R.D. Sturrock

doi:10.1016/0162-0134(88)85029-3

Resonance raman-spectroscopy of hemoglobin in intact-cells: A probe of oxygen-uptake by erythrocytes in rheumatoid arthritis

S. Hoey, D.H. Brown, A.A. McConnell, W.E. Smith, M. Marabani, R.D. Sturrock

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Resonance Raman spectra from intact viable erythrocytes can be used to study oxygen uptake in solution. In addition to changes in the oxidation state marker (v4), other bands due to the porphyrin ring (v3) and vinyl modes indicate subtle changes at oxygen pressures close to where the T/R change occurs. A comparison of whole cell and lysate spectra indicates a partial denaturation of hemoglobin on lysis. A simple smear technique is used to measure spectra from rheumatoid and normal blood. Results indicate a faster but less complete uptake of oxygen in cells from patients with rheumatoid arthritis than is the case in normal cell populations.

Original language	English
Pages (from-to)	189-199
Number of pages	10
Journal	Journal of Inorganic Biochemistry
Volume	34
Issue number	3
DOIs	https://doi.org/10.1016/0162-0134(88)85029-3
Publication status	Published - Nov 1988

Keywords

resonance raman-spectroscopy
hemoglobin
cells
oxygen-uptake
erythrocytes
rheumatoid arthritis

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10.1016/0162-0134(88)85029-3

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title = "Resonance raman-spectroscopy of hemoglobin in intact-cells: A probe of oxygen-uptake by erythrocytes in rheumatoid arthritis",

abstract = "Resonance Raman spectra from intact viable erythrocytes can be used to study oxygen uptake in solution. In addition to changes in the oxidation state marker (v4), other bands due to the porphyrin ring (v3) and vinyl modes indicate subtle changes at oxygen pressures close to where the T/R change occurs. A comparison of whole cell and lysate spectra indicates a partial denaturation of hemoglobin on lysis. A simple smear technique is used to measure spectra from rheumatoid and normal blood. Results indicate a faster but less complete uptake of oxygen in cells from patients with rheumatoid arthritis than is the case in normal cell populations.",

keywords = "resonance raman-spectroscopy, hemoglobin, cells, oxygen-uptake, erythrocytes, rheumatoid arthritis",

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year = "1988",

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T1 - Resonance raman-spectroscopy of hemoglobin in intact-cells: A probe of oxygen-uptake by erythrocytes in rheumatoid arthritis

AU - Hoey, S.

AU - Brown, D.H.

AU - McConnell, A.A.

AU - Smith, W.E.

AU - Marabani, M.

AU - Sturrock, R.D.

PY - 1988/11

Y1 - 1988/11

N2 - Resonance Raman spectra from intact viable erythrocytes can be used to study oxygen uptake in solution. In addition to changes in the oxidation state marker (v4), other bands due to the porphyrin ring (v3) and vinyl modes indicate subtle changes at oxygen pressures close to where the T/R change occurs. A comparison of whole cell and lysate spectra indicates a partial denaturation of hemoglobin on lysis. A simple smear technique is used to measure spectra from rheumatoid and normal blood. Results indicate a faster but less complete uptake of oxygen in cells from patients with rheumatoid arthritis than is the case in normal cell populations.

AB - Resonance Raman spectra from intact viable erythrocytes can be used to study oxygen uptake in solution. In addition to changes in the oxidation state marker (v4), other bands due to the porphyrin ring (v3) and vinyl modes indicate subtle changes at oxygen pressures close to where the T/R change occurs. A comparison of whole cell and lysate spectra indicates a partial denaturation of hemoglobin on lysis. A simple smear technique is used to measure spectra from rheumatoid and normal blood. Results indicate a faster but less complete uptake of oxygen in cells from patients with rheumatoid arthritis than is the case in normal cell populations.

KW - resonance raman-spectroscopy

KW - hemoglobin

KW - cells

KW - oxygen-uptake

KW - erythrocytes

KW - rheumatoid arthritis

UR - http://dx.doi.org/10.1016/0162-0134(88)85029-3

U2 - 10.1016/0162-0134(88)85029-3

DO - 10.1016/0162-0134(88)85029-3

M3 - Article

SN - 0162-0134

VL - 34

SP - 189

EP - 199

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

IS - 3

ER -

Resonance raman-spectroscopy of hemoglobin in intact-cells: A probe of oxygen-uptake by erythrocytes in rheumatoid arthritis

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