Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade

Gillian E Marshall; Julie A Russell; James O Tellez; Pardeep S Jhund; Susan Currie; John Dempster; Mark R Boyett; Kathleen A Kane; Andrew C Rankin; Antony J Workman

doi:10.1007/s00424-011-1061-z

Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade

Gillian E Marshall, Julie A Russell, James O Tellez, Pardeep S Jhund, Susan Currie, John Dempster, Mark R Boyett, Kathleen A Kane, Andrew C Rankin, Antony J Workman

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article

14 Citations (Scopus)

Abstract

Chronic β-adrenoceptor antagonist (β-blocker) treatment in patients is associated with a potentially anti-arrhythmic prolongation of the atrial action potential duration (APD), which may involve remodelling of repolarising K(+) currents. The aim of this study was to investigate the effects of chronic β-blockade on transient outward, sustained and inward rectifier K(+) currents (I(TO), I(KSUS) and I(K1)) in human atrial myocytes and on the expression of underlying ion channel subunits. Ion currents were recorded from human right atrial isolated myocytes using the whole-cell-patch clamp technique. Tissue mRNA and protein levels were measured using real time RT-PCR and Western blotting. Chronic β-blockade was associated with a 41% reduction in I(TO) density: 9.3 ± 0.8 (30 myocytes, 15 patients) vs 15.7 ± 1.1 pA/pF (32, 14), p

Original language	English
Pages (from-to)	537-548
Number of pages	12
Journal	Pflugers Archiv - European Journal of Physiology
Volume	463
Issue number	4
DOIs	https://doi.org/10.1007/s00424-011-1061-z
Publication status	Published - 2012

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Keywords

human atrial K+ currents
ion channel expression
pharmacology
therapeutic drugs

Cite this

Marshall, G. E., Russell, J. A., Tellez, J. O., Jhund, P. S., Currie, S., Dempster, J., ... Workman, A. J. (2012). Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade. Pflugers Archiv - European Journal of Physiology, 463(4), 537-548. https://doi.org/10.1007/s00424-011-1061-z

Marshall, Gillian E ; Russell, Julie A ; Tellez, James O ; Jhund, Pardeep S ; Currie, Susan ; Dempster, John ; Boyett, Mark R ; Kane, Kathleen A ; Rankin, Andrew C ; Workman, Antony J. / Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade. In: Pflugers Archiv - European Journal of Physiology. 2012 ; Vol. 463, No. 4. pp. 537-548.

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abstract = "Chronic β-adrenoceptor antagonist (β-blocker) treatment in patients is associated with a potentially anti-arrhythmic prolongation of the atrial action potential duration (APD), which may involve remodelling of repolarising K(+) currents. The aim of this study was to investigate the effects of chronic β-blockade on transient outward, sustained and inward rectifier K(+) currents (I(TO), I(KSUS) and I(K1)) in human atrial myocytes and on the expression of underlying ion channel subunits. Ion currents were recorded from human right atrial isolated myocytes using the whole-cell-patch clamp technique. Tissue mRNA and protein levels were measured using real time RT-PCR and Western blotting. Chronic β-blockade was associated with a 41{\%} reduction in I(TO) density: 9.3 ± 0.8 (30 myocytes, 15 patients) vs 15.7 ± 1.1 pA/pF (32, 14), p ",

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Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade. / Marshall, Gillian E; Russell, Julie A; Tellez, James O; Jhund, Pardeep S; Currie, Susan; Dempster, John; Boyett, Mark R; Kane, Kathleen A; Rankin, Andrew C; Workman, Antony J.

In: Pflugers Archiv - European Journal of Physiology, Vol. 463, No. 4, 2012, p. 537-548.

Research output: Contribution to journal › Article

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AU - Russell, Julie A

AU - Tellez, James O

AU - Jhund, Pardeep S

AU - Currie, Susan

AU - Dempster, John

AU - Boyett, Mark R

AU - Kane, Kathleen A

AU - Rankin, Andrew C

AU - Workman, Antony J

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AB - Chronic β-adrenoceptor antagonist (β-blocker) treatment in patients is associated with a potentially anti-arrhythmic prolongation of the atrial action potential duration (APD), which may involve remodelling of repolarising K(+) currents. The aim of this study was to investigate the effects of chronic β-blockade on transient outward, sustained and inward rectifier K(+) currents (I(TO), I(KSUS) and I(K1)) in human atrial myocytes and on the expression of underlying ion channel subunits. Ion currents were recorded from human right atrial isolated myocytes using the whole-cell-patch clamp technique. Tissue mRNA and protein levels were measured using real time RT-PCR and Western blotting. Chronic β-blockade was associated with a 41% reduction in I(TO) density: 9.3 ± 0.8 (30 myocytes, 15 patients) vs 15.7 ± 1.1 pA/pF (32, 14), p

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10.1007/s00424-011-1061-z