Regulation of store-operated Ca2+ entry in pulmonary artery smooth muscle cells

S.P. McElroy, R.M. Drummond, A.M. Gurney

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Store-operated Ca2+ entry (SOCE) is an important mechanism for Ca2+ influx in smooth muscle cells; however the activation and regulation of this influx pathway are incompletely understood. In the present study we have examined the effect of several protein kinases in regulating SOCE in pulmonary artery smooth muscle cells (PASMCs) of the rat. Inhibition of protein kinase C with chelerythrine (3 μM) potentiated SOCE by 47 ± 2%, while the tyrosine kinase inhibitors genistein (100 μM) and tyrphostin 23 (100 μM) caused a significant reduction in SOCE of 55 ± 9% and 43 ± 7%, respectively. It has been proposed that Ca2+-insensitive phospholipase A2 (iPLA2) is involved in the activation of SOCE in many different cell types. The iPLA2 inhibitor, bromoenol lactone had no effect on SOCE, suggesting that this mechanism was not involved in the activation of the pathway. The calmodulin antagonists, calmidazolium (CMZ) (10 μM) and W-7 (10 μM) appeared to potentiate SOCE in PASMCs. Further investigation established that CMZ was actually activating a Ca2+ influx pathway that was independent of the filling state of the sarcoplasmic reticulum. The CMZ-activated Ca2+ influx was blocked by Gd3+ (10 μM), but unaffected by 2-APB (75 μM), indicating a pharmacological profile distinct from the classical SOCE pathway.
Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalCell Calcium
Volume46
Issue number2
DOIs
Publication statusPublished - Aug 2009

Fingerprint

calmidazolium
Pulmonary Artery
Smooth Muscle Myocytes
TYK2 Kinase
Genistein
Phospholipases A2
Sarcoplasmic Reticulum
Calmodulin
Protein Kinases
Protein Kinase C
Pharmacology

Keywords

  • pulmonary artery
  • smooth muscle
  • store-operated Ca2+ entry
  • protein kinase C
  • tyrosine kinase
  • calmodulin
  • Ca2+ independent phospholipase A(2)
  • ca2+-independent phospholipase a(2)
  • protein-kinase-c
  • capacitative calcium-entry
  • inositol 1
  • 4
  • 5-trisphosphate receptors
  • portal-vein myocytes
  • bromoenol lactone
  • trpc channels
  • crac channel
  • intravenous anesthetics

Cite this

McElroy, S.P. ; Drummond, R.M. ; Gurney, A.M. / Regulation of store-operated Ca2+ entry in pulmonary artery smooth muscle cells. In: Cell Calcium. 2009 ; Vol. 46, No. 2. pp. 99-106.
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Regulation of store-operated Ca2+ entry in pulmonary artery smooth muscle cells. / McElroy, S.P.; Drummond, R.M.; Gurney, A.M.

In: Cell Calcium, Vol. 46, No. 2, 08.2009, p. 99-106.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regulation of store-operated Ca2+ entry in pulmonary artery smooth muscle cells

AU - McElroy, S.P.

AU - Drummond, R.M.

AU - Gurney, A.M.

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AB - Store-operated Ca2+ entry (SOCE) is an important mechanism for Ca2+ influx in smooth muscle cells; however the activation and regulation of this influx pathway are incompletely understood. In the present study we have examined the effect of several protein kinases in regulating SOCE in pulmonary artery smooth muscle cells (PASMCs) of the rat. Inhibition of protein kinase C with chelerythrine (3 μM) potentiated SOCE by 47 ± 2%, while the tyrosine kinase inhibitors genistein (100 μM) and tyrphostin 23 (100 μM) caused a significant reduction in SOCE of 55 ± 9% and 43 ± 7%, respectively. It has been proposed that Ca2+-insensitive phospholipase A2 (iPLA2) is involved in the activation of SOCE in many different cell types. The iPLA2 inhibitor, bromoenol lactone had no effect on SOCE, suggesting that this mechanism was not involved in the activation of the pathway. The calmodulin antagonists, calmidazolium (CMZ) (10 μM) and W-7 (10 μM) appeared to potentiate SOCE in PASMCs. Further investigation established that CMZ was actually activating a Ca2+ influx pathway that was independent of the filling state of the sarcoplasmic reticulum. The CMZ-activated Ca2+ influx was blocked by Gd3+ (10 μM), but unaffected by 2-APB (75 μM), indicating a pharmacological profile distinct from the classical SOCE pathway.

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KW - bromoenol lactone

KW - trpc channels

KW - crac channel

KW - intravenous anesthetics

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DO - 10.1016/j.ceca.2009.05.006

M3 - Article

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JO - Cell Calcium

JF - Cell Calcium

SN - 0143-4160

IS - 2

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