Reflux precipitation polymerization: a new synthetic insight in molecular imprinting at high temperature

Xiantao Shen, Chuixiu Huang, Sudhirkumar Shinde, Magdalena Switnicka-Plak, Peter Cormack, Börje Sellergren

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
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Synthesis of uniform molecularly imprinted polymer (MIP) microspheres (MSs) using distillation precipitation polymerization (DPP) at high temperature has attracted great interest in the field of molecular imprinting. However,there were still some shortcomings in this method. In this work, to create uniform MIP MSs in a short time and to demonstrate the effects of high temperature on imprinting performance, a new precipitation polymerization method (reflux precipitation polymerization, RPP) was used for the first time to fabricate MIP MSs in this study. The SEM images of the polymeric MSs indicate the presence of template molecules could improve the particle morphology and size uniformity. The specific molecular recognition of the monodispersed MIP MSs was confirmed by fluorescence measurement and HPLC-UV analysis. The binding behavior of the MIP MSs was simulated using the heterogeneous Freundlich isotherm, which shows that the MIP MSs produced by the RPP possess compatible selectivity in comparison with those by traditional PP method. It is noted that, for the first time, we demonstrated that molecular imprinting at high temperature was only successful when electrostatic interactions played important roles in the imprinting process.

Original languageEnglish
Pages (from-to)81491-81499
Number of pages9
JournalRSC Advances
Issue number85
Early online date22 Aug 2016
Publication statusE-pub ahead of print - 22 Aug 2016


  • molecularly imprinted polymer microspheres
  • precipitation polymerization
  • molecular imprinting
  • reflux precipitation polymerization
  • particle morphology
  • size uniformity
  • binding behavior


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