Reduction of acetylcholine sensitivity of chick muscle in culture by myasthenia gravis serum

A. L. Harvey, T. Barkas, R. Harrison, G. G. Lunt, F. A. Stephenson, R. H. Teague

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sera from seven patients with myasthenia gravis and from six control subjects were tested for the presence of anti-acetylcholine receptor antibodies and for the ability to reduce acetylcholine sensitivity of embryonic chick skeletal muscle cells in culture. Anti-receptor antibodies were found in all but one of the myasthenic samples and in none of the control samples. The average reduction in sensitivity caused by the myasthenic sera was 89 ± 4%, compared with an average reduction of 15 ± 7% caused by the control sera. A sample taken from a patient during myasthenic crisis was found to have less blocking activity and less anti-receptor antibody than a sample from the same patient after recovery.

LanguageEnglish
Pages411-416
Number of pages6
JournalClinical and Experimental Immunology
Volume34
Issue number3
Publication statusPublished - 1 Dec 1978

Fingerprint

Myasthenia Gravis
Muscle Weakness
Acetylcholine
Muscles
Anti-Idiotypic Antibodies
Serum
Cholinergic Receptors
Muscle Cells
Skeletal Muscle
Cell Culture Techniques
Antibodies

Keywords

  • cholinergic receptor antibody
  • autoimmune disease
  • cell culture
  • in vitro study
  • major clinical study
  • muscle cell
  • myasthenia gravis
  • acetylcholine

Cite this

Harvey, A. L., Barkas, T., Harrison, R., Lunt, G. G., Stephenson, F. A., & Teague, R. H. (1978). Reduction of acetylcholine sensitivity of chick muscle in culture by myasthenia gravis serum. Clinical and Experimental Immunology, 34(3), 411-416.
Harvey, A. L. ; Barkas, T. ; Harrison, R. ; Lunt, G. G. ; Stephenson, F. A. ; Teague, R. H. / Reduction of acetylcholine sensitivity of chick muscle in culture by myasthenia gravis serum. In: Clinical and Experimental Immunology. 1978 ; Vol. 34, No. 3. pp. 411-416.
@article{f83bad425c454663abb2db3b0de382b8,
title = "Reduction of acetylcholine sensitivity of chick muscle in culture by myasthenia gravis serum",
abstract = "Sera from seven patients with myasthenia gravis and from six control subjects were tested for the presence of anti-acetylcholine receptor antibodies and for the ability to reduce acetylcholine sensitivity of embryonic chick skeletal muscle cells in culture. Anti-receptor antibodies were found in all but one of the myasthenic samples and in none of the control samples. The average reduction in sensitivity caused by the myasthenic sera was 89 ± 4{\%}, compared with an average reduction of 15 ± 7{\%} caused by the control sera. A sample taken from a patient during myasthenic crisis was found to have less blocking activity and less anti-receptor antibody than a sample from the same patient after recovery.",
keywords = "cholinergic receptor antibody, autoimmune disease, cell culture, in vitro study, major clinical study, muscle cell, myasthenia gravis, acetylcholine",
author = "Harvey, {A. L.} and T. Barkas and R. Harrison and Lunt, {G. G.} and Stephenson, {F. A.} and Teague, {R. H.}",
year = "1978",
month = "12",
day = "1",
language = "English",
volume = "34",
pages = "411--416",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
number = "3",

}

Harvey, AL, Barkas, T, Harrison, R, Lunt, GG, Stephenson, FA & Teague, RH 1978, 'Reduction of acetylcholine sensitivity of chick muscle in culture by myasthenia gravis serum' Clinical and Experimental Immunology, vol. 34, no. 3, pp. 411-416.

Reduction of acetylcholine sensitivity of chick muscle in culture by myasthenia gravis serum. / Harvey, A. L.; Barkas, T.; Harrison, R.; Lunt, G. G.; Stephenson, F. A.; Teague, R. H.

In: Clinical and Experimental Immunology, Vol. 34, No. 3, 01.12.1978, p. 411-416.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reduction of acetylcholine sensitivity of chick muscle in culture by myasthenia gravis serum

AU - Harvey, A. L.

AU - Barkas, T.

AU - Harrison, R.

AU - Lunt, G. G.

AU - Stephenson, F. A.

AU - Teague, R. H.

PY - 1978/12/1

Y1 - 1978/12/1

N2 - Sera from seven patients with myasthenia gravis and from six control subjects were tested for the presence of anti-acetylcholine receptor antibodies and for the ability to reduce acetylcholine sensitivity of embryonic chick skeletal muscle cells in culture. Anti-receptor antibodies were found in all but one of the myasthenic samples and in none of the control samples. The average reduction in sensitivity caused by the myasthenic sera was 89 ± 4%, compared with an average reduction of 15 ± 7% caused by the control sera. A sample taken from a patient during myasthenic crisis was found to have less blocking activity and less anti-receptor antibody than a sample from the same patient after recovery.

AB - Sera from seven patients with myasthenia gravis and from six control subjects were tested for the presence of anti-acetylcholine receptor antibodies and for the ability to reduce acetylcholine sensitivity of embryonic chick skeletal muscle cells in culture. Anti-receptor antibodies were found in all but one of the myasthenic samples and in none of the control samples. The average reduction in sensitivity caused by the myasthenic sera was 89 ± 4%, compared with an average reduction of 15 ± 7% caused by the control sera. A sample taken from a patient during myasthenic crisis was found to have less blocking activity and less anti-receptor antibody than a sample from the same patient after recovery.

KW - cholinergic receptor antibody

KW - autoimmune disease

KW - cell culture

KW - in vitro study

KW - major clinical study

KW - muscle cell

KW - myasthenia gravis

KW - acetylcholine

UR - http://www.scopus.com/inward/record.url?scp=0018218830&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 411

EP - 416

JO - Clinical and Experimental Immunology

T2 - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 3

ER -