Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

D. Stevenson, C. Morgan, L.A. McLellan, M.H. Grant

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cryopreservation of hepatocytes as monolayers circumvents the problems of low post-thaw recovery and attachment in culture obtained with hepatocytes frozen in suspension. We have shown that cytochrome P450 dependent generation of reactive metabolites takes place readily in these cells (Stevenson et al., 2004), and in the present study the reduced glutathione (GSH) levels and the expression of the catalytic subunit of glutamate cysteine ligase (GCL) and of glutathione synthetase (GSS) have been investigated.
Original languageEnglish
Pages (from-to)100-101
Number of pages1
JournalToxicology
Volume231
Issue number2-3
DOIs
Publication statusPublished - 2007

Fingerprint

Glutathione
Hepatocytes
Monolayers
Glutathione Synthase
Glutamate-Cysteine Ligase
Cryopreservation
Enzymes
Cytochromes
Metabolites
Catalytic Domain
Suspensions
Recovery

Keywords

  • cryopreserved hepatocyte monolayers
  • reduced glutathione
  • glutathione synthesis
  • expression of glutathione synthetase

Cite this

@article{5faddd8e8d384712a56853ae12fbcaab,
title = "Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures",
abstract = "Cryopreservation of hepatocytes as monolayers circumvents the problems of low post-thaw recovery and attachment in culture obtained with hepatocytes frozen in suspension. We have shown that cytochrome P450 dependent generation of reactive metabolites takes place readily in these cells (Stevenson et al., 2004), and in the present study the reduced glutathione (GSH) levels and the expression of the catalytic subunit of glutamate cysteine ligase (GCL) and of glutathione synthetase (GSS) have been investigated.",
keywords = "cryopreserved hepatocyte monolayers, reduced glutathione, glutathione synthesis, expression of glutathione synthetase",
author = "D. Stevenson and C. Morgan and L.A. McLellan and M.H. Grant",
note = "See also journal article: http://strathprints.strath.ac.uk/28452/",
year = "2007",
doi = "10.1016/j.tox.2006.11.023",
language = "English",
volume = "231",
pages = "100--101",
journal = "Toxicology",
issn = "0300-483X",
number = "2-3",

}

Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures. / Stevenson, D.; Morgan, C.; McLellan, L.A.; Grant, M.H.

In: Toxicology, Vol. 231, No. 2-3, 2007, p. 100-101.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

AU - Stevenson, D.

AU - Morgan, C.

AU - McLellan, L.A.

AU - Grant, M.H.

N1 - See also journal article: http://strathprints.strath.ac.uk/28452/

PY - 2007

Y1 - 2007

N2 - Cryopreservation of hepatocytes as monolayers circumvents the problems of low post-thaw recovery and attachment in culture obtained with hepatocytes frozen in suspension. We have shown that cytochrome P450 dependent generation of reactive metabolites takes place readily in these cells (Stevenson et al., 2004), and in the present study the reduced glutathione (GSH) levels and the expression of the catalytic subunit of glutamate cysteine ligase (GCL) and of glutathione synthetase (GSS) have been investigated.

AB - Cryopreservation of hepatocytes as monolayers circumvents the problems of low post-thaw recovery and attachment in culture obtained with hepatocytes frozen in suspension. We have shown that cytochrome P450 dependent generation of reactive metabolites takes place readily in these cells (Stevenson et al., 2004), and in the present study the reduced glutathione (GSH) levels and the expression of the catalytic subunit of glutamate cysteine ligase (GCL) and of glutathione synthetase (GSS) have been investigated.

KW - cryopreserved hepatocyte monolayers

KW - reduced glutathione

KW - glutathione synthesis

KW - expression of glutathione synthetase

UR - http://strathprints.strath.ac.uk/28452/

UR - http://dx.doi.org/10.1016/j.tox.2006.11.023

U2 - 10.1016/j.tox.2006.11.023

DO - 10.1016/j.tox.2006.11.023

M3 - Article

VL - 231

SP - 100

EP - 101

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 2-3

ER -