Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

D. Stevenson, C. Morgan, L.A. McLellan, M.H. Grant

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cryopreservation of hepatocytes as monolayers circumvents the problems of low post-thaw recovery and attachment in culture obtained with hepatocytes frozen in suspension. We have shown that cytochrome P450 dependent generation of reactive metabolites takes place readily in these cells (Stevenson et al., 2004), and in the present study the reduced glutathione (GSH) levels and the expression of the catalytic subunit of glutamate cysteine ligase (GCL) and of glutathione synthetase (GSS) have been investigated.
Original languageEnglish
Pages (from-to)100-101
Number of pages1
JournalToxicology
Volume231
Issue number2-3
DOIs
Publication statusPublished - 2007

Keywords

  • cryopreserved hepatocyte monolayers
  • reduced glutathione
  • glutathione synthesis
  • expression of glutathione synthetase

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