Receptor usage by the Acanthocheilonema viteae-derived immunomodulator, ES-62

William Harnett, Helen Goodridge, Janet Allen, Margaret Harnett

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Abstract

ES-62 is an immunomodulatory phosphorylcholine (PC)-containing glycoprotein secreted by the rodent filarial nematode Acanthocheilonema viteae. Previously, the use of knockout mice has revealed the effects of ES-62 on macrophages and dendritic cells to be dependent on TLR4. However, it is possible that ES-62
may interact with additional proteins on the surfaces of target cells and hence that cells may vary with respect to receptor usage. In this study, we identified by molecular weight, proteins that interact with ES-62 and found differences amongst the immune system cells studied. Thus, whereas lymphocytes appear
to have two major interacting proteins of 135 and 82 kDa, U937 monocytes only contain an ES-62-binding protein of the latter molecular weight. Binding to the proteins on B cells and U937 cells wasblocked by PC, suggesting a critical role for this ES-62 moiety in facilitating interaction. Finally, ES-62 binding is followed by internalization in both macrophages and B cells but only in the former was
absence of TLR4 found to block internalization. These findings are consistent with differences in receptor usage by ES-62 amongst different cell-types.
Original languageEnglish
Number of pages6
JournalExperimental Parasitology
Early online date8 Sep 2011
DOIs
Publication statusPublished - 30 Sep 2012

Keywords

  • acanthocheilonema
  • anthocheilonema viteae
  • filarial
  • immunomodulator
  • receptor usage
  • macrophage
  • protein

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