Recent advances in single-cell subcellular sampling

Annie Sahota, Anthony Monteza Cabrejos, Zoe Kwan, Binoy Paulose Nadappuram, Aleksandar P. Ivanov, Joshua B. Edel

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Recent innovations in single-cell technologies have opened up exciting possibilities for profiling the omics of individual cells. Minimally invasive analysis tools that probe and remove the contents of living cells enable cells to remain in their standard microenvironment with little impact on their viability. This negates the requirement of lysing cells to access their contents, an advancement from previous single-cell manipulation methods. These novel methods have the potential to be used for dynamic studies on single cells, with many already providing high intracellular spatial resolution. In this article, we highlight key technological advances that aim to remove the contents of living cells for downstream analysis. Recent applications of these techniques are reviewed, along with their current limitations. We also propose recommendations for expanding the scope of these technologies to achieve comprehensive single-cell tracking in the future, anticipating the discovery of subcellular mechanisms and novel therapeutic targets and treatments, ultimately transforming the fields of spatial transcriptomics and personalised medicine.
Original languageEnglish
Pages (from-to)5312-5328
Number of pages17
JournalChemical Communications
Issue number36
Early online date3 Apr 2023
Publication statusE-pub ahead of print - 3 Apr 2023


  • single-cell technologies
  • nanostraws
  • atomic force microscopy-based FluidFM
  • dielectrophoretic systems


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