Real-time imaging of the cellular interactions underlying tolerance, priming, and responses to infection

J.M. Brewer, P. Garside

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Much of what we understand about the anatomy and architecture of the immune system was revealed through exquisite experiments performed in the 1950s-1970s. These studies identified the role that anatomy played in a number of fundamental immunological phenomena including recirculation, induction of immune priming or tolerance, and the interactions of T and B cells. The recent resurgence of interest in the role of immune architecture and anatomy in basic immunological phenomena is almost entirely due to technological developments in identifying and tracking cells in vivo, not least through the ability to do this dynamically, in real time through the application of multiphoton microscopy. Here we outline the background to our own studies applying multiphoton microscopy to analysis of immune priming and tolerance, the role of adjuvants, T- and B-cell interactions, and the application of these studies in infectious and inflammatory diseases. We then describe the impact that real time in vivo imaging has had on these areas. Finally, we engage in some 'crystal ball gazing' to look at what developments in imaging are likely to occur, why they are important, and what further information these approaches may distill regarding the development of the immune response.
Original languageEnglish
Pages (from-to)130-146
Number of pages16
JournalImmunological Reviews
Volume221
Issue number1
DOIs
Publication statusPublished - 2008

Fingerprint

Anatomy
Microscopy
B-Lymphocytes
Infection
Cell Tracking
Immune Tolerance
Cell Communication
Communicable Diseases
Immune System
T-Lymphocytes

Keywords

  • imaging
  • tolerance
  • priming
  • adjuvants
  • arthritis
  • malaria
  • biophotonics

Cite this

@article{827cf1e877b24abd991465e341952ba7,
title = "Real-time imaging of the cellular interactions underlying tolerance, priming, and responses to infection",
abstract = "Much of what we understand about the anatomy and architecture of the immune system was revealed through exquisite experiments performed in the 1950s-1970s. These studies identified the role that anatomy played in a number of fundamental immunological phenomena including recirculation, induction of immune priming or tolerance, and the interactions of T and B cells. The recent resurgence of interest in the role of immune architecture and anatomy in basic immunological phenomena is almost entirely due to technological developments in identifying and tracking cells in vivo, not least through the ability to do this dynamically, in real time through the application of multiphoton microscopy. Here we outline the background to our own studies applying multiphoton microscopy to analysis of immune priming and tolerance, the role of adjuvants, T- and B-cell interactions, and the application of these studies in infectious and inflammatory diseases. We then describe the impact that real time in vivo imaging has had on these areas. Finally, we engage in some 'crystal ball gazing' to look at what developments in imaging are likely to occur, why they are important, and what further information these approaches may distill regarding the development of the immune response.",
keywords = "imaging, tolerance, priming, adjuvants, arthritis, malaria, biophotonics",
author = "J.M. Brewer and P. Garside",
year = "2008",
doi = "10.1111/j.1600-065X.2008.00587.x",
language = "English",
volume = "221",
pages = "130--146",
journal = "Immunological Reviews",
issn = "0105-2896",
number = "1",

}

Real-time imaging of the cellular interactions underlying tolerance, priming, and responses to infection. / Brewer, J.M.; Garside, P.

In: Immunological Reviews, Vol. 221, No. 1, 2008, p. 130-146.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Real-time imaging of the cellular interactions underlying tolerance, priming, and responses to infection

AU - Brewer, J.M.

AU - Garside, P.

PY - 2008

Y1 - 2008

N2 - Much of what we understand about the anatomy and architecture of the immune system was revealed through exquisite experiments performed in the 1950s-1970s. These studies identified the role that anatomy played in a number of fundamental immunological phenomena including recirculation, induction of immune priming or tolerance, and the interactions of T and B cells. The recent resurgence of interest in the role of immune architecture and anatomy in basic immunological phenomena is almost entirely due to technological developments in identifying and tracking cells in vivo, not least through the ability to do this dynamically, in real time through the application of multiphoton microscopy. Here we outline the background to our own studies applying multiphoton microscopy to analysis of immune priming and tolerance, the role of adjuvants, T- and B-cell interactions, and the application of these studies in infectious and inflammatory diseases. We then describe the impact that real time in vivo imaging has had on these areas. Finally, we engage in some 'crystal ball gazing' to look at what developments in imaging are likely to occur, why they are important, and what further information these approaches may distill regarding the development of the immune response.

AB - Much of what we understand about the anatomy and architecture of the immune system was revealed through exquisite experiments performed in the 1950s-1970s. These studies identified the role that anatomy played in a number of fundamental immunological phenomena including recirculation, induction of immune priming or tolerance, and the interactions of T and B cells. The recent resurgence of interest in the role of immune architecture and anatomy in basic immunological phenomena is almost entirely due to technological developments in identifying and tracking cells in vivo, not least through the ability to do this dynamically, in real time through the application of multiphoton microscopy. Here we outline the background to our own studies applying multiphoton microscopy to analysis of immune priming and tolerance, the role of adjuvants, T- and B-cell interactions, and the application of these studies in infectious and inflammatory diseases. We then describe the impact that real time in vivo imaging has had on these areas. Finally, we engage in some 'crystal ball gazing' to look at what developments in imaging are likely to occur, why they are important, and what further information these approaches may distill regarding the development of the immune response.

KW - imaging

KW - tolerance

KW - priming

KW - adjuvants

KW - arthritis

KW - malaria

KW - biophotonics

UR - http://dx.doi.org/10.1111/j.1600-065X.2008.00587.x

U2 - 10.1111/j.1600-065X.2008.00587.x

DO - 10.1111/j.1600-065X.2008.00587.x

M3 - Article

VL - 221

SP - 130

EP - 146

JO - Immunological Reviews

JF - Immunological Reviews

SN - 0105-2896

IS - 1

ER -