Real-time evaluation of aggregation using confocal imaging and image analysis tools

Zahra Hamrang; Egor Zindy; David Clarke; Alain Pluen

doi:10.1039/C3AN01693E

Real-time evaluation of aggregation using confocal imaging and image analysis tools

Zahra Hamrang, Egor Zindy, David Clarke, Alain Pluen

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster Image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.

Original language	English
Pages (from-to)	564-568
Number of pages	4
Journal	Analyst
Volume	139
Issue number	3
Early online date	20 Nov 2013
DOIs	https://doi.org/10.1039/C3AN01693E
Publication status	Published - 7 Feb 2014

Keywords

confocal imaging
in situ
BSA monomers
Spatial Intensity Distribution Analysis (SpIDA)
Raster Image Correlation Spectroscopy (RICS)

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10.1039/C3AN01693E

Cite this

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abstract = "Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster Image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.",

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AU - Clarke, David

AU - Pluen, Alain

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AB - Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster Image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.

KW - confocal imaging

KW - in situ

KW - BSA monomers

KW - Spatial Intensity Distribution Analysis (SpIDA)

KW - Raster Image Correlation Spectroscopy (RICS)

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JO - Analyst

JF - Analyst

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ER -

Real-time evaluation of aggregation using confocal imaging and image analysis tools

Abstract

Keywords

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