Real-time evaluation of aggregation using confocal imaging and image analysis tools

Zahra Hamrang, Egor Zindy, David Clarke, Alain Pluen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster Image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.
Original languageEnglish
Pages (from-to)564-568
Number of pages4
JournalAnalyst
Volume139
Issue number3
Early online date20 Nov 2013
DOIs
Publication statusPublished - 7 Feb 2014

Keywords

  • confocal imaging
  • in situ
  • BSA monomers
  • Spatial Intensity Distribution Analysis (SpIDA)
  • Raster Image Correlation Spectroscopy (RICS)

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