Rapid genotyping of Shigella sonnei using multiplex high resolution melting

Waleed Mazi; Vartul Sangal; Amir Saeed; Gunnar   Sandstom; François-Xavier  Weill; Jun Yu

doi:10.1128/JCM.00874-15

Rapid genotyping of Shigella sonnei using multiplex high resolution melting

Waleed Mazi, Vartul Sangal, Amir Saeed, Gunnar Sandstom, François-Xavier Weill , Jun Yu

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Shigella sonnei, an emerging global cause of shigellosis, consists of four distinct lineages and the current pandemic involves several geographically associated, multidrug- resistant clones that belong to lineage III (1-3). A typing scheme based on high resolution melting (HRM) of six chromosomal single nucleotide polymorphisms (SNPs) has been described to identify all lineages/sub-lineages (4).

Original language	English
Article number	JCM.00874-15
Pages (from-to)	1-2
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	53
Issue number	7
Early online date	29 Apr 2015
DOIs	https://doi.org/10.1128/JCM.00874-15
Publication status	E-pub ahead of print - 29 Apr 2015

Keywords

high resolution melting
shgiella sonnei
melting temperature
single nucleotide polymorphisms

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10.1128/JCM.00874-15

Cite this

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abstract = "Shigella sonnei, an emerging global cause of shigellosis, consists of four distinct lineages and the current pandemic involves several geographically associated, multidrug- resistant clones that belong to lineage III (1-3). A typing scheme based on high resolution melting (HRM) of six chromosomal single nucleotide polymorphisms (SNPs) has been described to identify all lineages/sub-lineages (4). ",

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Rapid genotyping of Shigella sonnei using multiplex high resolution melting

Abstract

Keywords

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Global phylogeny of Shigella sonnei from limited single nucleotide polymorphisms and development of a rapid and cost-effective SNP-typing scheme for strain identification by high resolution melting: rapid SNP-typing S. sonnei

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