Rapid detection of wound pathogen Proteus mirabilis using disposable electrochemical sensors

Research output: Contribution to conferenceProceedingpeer-review

10 Downloads (Pure)

Abstract

The detection of infection in clinical practice is a time consuming and laborious process. The ability to monitor infection status in real time, for example in wounds, would enable earlier intervention and improved prognosis. This study describes the real time electrochemical detection of the clinically important pathogen Proteus mirabilis. Using impedance spectroscopy in conjunction with a normalisation approach, the growth of P. mirabilis in LB medium was detected 1 hour after sample inoculation at a cell concentration of 7.4 x106 CFU/mL. Furthermore, a significant decrease in charge transfer resistance arose over the 24 hour growth period (p = 0.009), modelled using a simple equivalent circuit. Additional experiments performed in 0.9% w/v NaCl (where growth was inhibited) indicated that processes facilitated by the organism’s metabolism and growth dominated the impedance response in LB medium. A simulated wound fluid was used to explore a more complex environment, and similar changes to normalised impedance were observed. The ability of these low cost sensors to rapidly detect P. mirabilis highlights their potential for adoption into point-of-care infection monitoring devices.
Original languageEnglish
Number of pages8
DOIs
Publication statusPublished - 31 Jul 2021
Event7th World Congress on Electrical Engineering and Computer Systems Science - Virtual, Prague, Czech Republic
Duration: 29 Jul 202131 Jul 2021
Conference number: 7
https://eecss.org/

Conference

Conference7th World Congress on Electrical Engineering and Computer Systems Science
Abbreviated titleEECSS'21
Country/TerritoryCzech Republic
CityPrague
Period29/07/2131/07/21
Internet address

Keywords

  • impedance spectroscopy
  • infection
  • rapid diagnostics
  • screen printing
  • bacterial detection
  • Proteus mirabilis

Fingerprint

Dive into the research topics of 'Rapid detection of wound pathogen Proteus mirabilis using disposable electrochemical sensors'. Together they form a unique fingerprint.
  • Best Paper Award

    Hannah, Aiden (Recipient), Aug 2021

    Prize: Prize (including medals and awards)

    File

Cite this