Abstract
| Language | English |
|---|---|
| Pages | 170-175 |
| Number of pages | 6 |
| Journal | Analyst |
| Volume | 134 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- rapid cell mapping
- nanoparticles
- SERRS
- pharmacology
- biomedical sciences
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Rapid cell mapping using nanoparticles and SERRS. / Stokes, Robert J.; McKenzie, Fiona; McFarlane, Emma; Ricketts, Alastair; Tetley, L.; Faulds, Karen; Alexander, J.; Graham, Duncan.
In: Analyst, Vol. 134, No. 1, 2009, p. 170-175.Research output: Contribution to journal › Article
TY - JOUR
T1 - Rapid cell mapping using nanoparticles and SERRS
AU - Stokes, Robert J.
AU - McKenzie, Fiona
AU - McFarlane, Emma
AU - Ricketts, Alastair
AU - Tetley, L.
AU - Faulds, Karen
AU - Alexander, J.
AU - Graham, Duncan
PY - 2009
Y1 - 2009
N2 - Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
AB - Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
KW - rapid cell mapping
KW - nanoparticles
KW - SERRS
KW - pharmacology
KW - biomedical sciences
U2 - 10.1039/b815117b
DO - 10.1039/b815117b
M3 - Article
VL - 134
SP - 170
EP - 175
JO - Analyst
T2 - Analyst
JF - Analyst
SN - 0003-2654
IS - 1
ER -