Rapid cell mapping using nanoparticles and SERRS

Robert J. Stokes, Fiona McKenzie, Emma McFarlane, Alastair Ricketts, L. Tetley, Karen Faulds, J. Alexander, Duncan Graham

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
Original languageEnglish
Pages (from-to)170-175
Number of pages6
JournalAnalyst
Volume134
Issue number1
DOIs
Publication statusPublished - 2009

Keywords

  • rapid cell mapping
  • nanoparticles
  • SERRS
  • pharmacology
  • biomedical sciences

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    Cite this

    Stokes, R. J., McKenzie, F., McFarlane, E., Ricketts, A., Tetley, L., Faulds, K., Alexander, J., & Graham, D. (2009). Rapid cell mapping using nanoparticles and SERRS. Analyst, 134(1), 170-175. https://doi.org/10.1039/b815117b