Rapid cell mapping using nanoparticles and SERRS

Robert J. Stokes; Fiona McKenzie; Emma McFarlane; Alastair Ricketts; L. Tetley; Karen Faulds; J. Alexander; Duncan Graham

doi:10.1039/b815117b

Rapid cell mapping using nanoparticles and SERRS

Robert J. Stokes, Fiona McKenzie, Emma McFarlane, Alastair Ricketts, L. Tetley, Karen Faulds, J. Alexander, Duncan Graham

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.

Original language	English
Pages (from-to)	170-175
Number of pages	6
Journal	Analyst
Volume	134
Issue number	1
DOIs	https://doi.org/10.1039/b815117b
Publication status	Published - 2009

Keywords

rapid cell mapping
nanoparticles
SERRS
pharmacology
biomedical sciences

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10.1039/b815117b

2 Finished

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, D., Dawson, M., Faulds, K., Girkin, J., Graham, D., Martin, R., O'Donnell, K., Rolinski, O., Smith, W. & Wynne, K.
Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)
1/08/08 → 31/01/12
Project: Research
OPTICAL SPECTROSCOPIES FOR IMMUNO-BIOMEDICAL APPLICATIONS
Graham, D., Alexander, J., Birch, D., Faulds, K., Garside, P. & Smith, W.
EPSRC (Engineering and Physical Sciences Research Council)
1/08/06 → 31/01/12
Project: Research

Cite this

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abstract = "Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.",

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AU - Stokes, Robert J.

AU - McKenzie, Fiona

AU - McFarlane, Emma

AU - Ricketts, Alastair

AU - Tetley, L.

AU - Faulds, Karen

AU - Alexander, J.

AU - Graham, Duncan

PY - 2009

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AB - Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.

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KW - nanoparticles

KW - SERRS

KW - pharmacology

KW - biomedical sciences

U2 - 10.1039/b815117b

DO - 10.1039/b815117b

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SP - 170

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JO - Analyst

JF - Analyst

SN - 0003-2654

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Rapid cell mapping using nanoparticles and SERRS

Abstract

Keywords

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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

OPTICAL SPECTROSCOPIES FOR IMMUNO-BIOMEDICAL APPLICATIONS

Cite this