Projects per year
Abstract
Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
| Original language | English |
|---|---|
| Pages (from-to) | 170-175 |
| Number of pages | 6 |
| Journal | Analyst |
| Volume | 134 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 |
Keywords
- rapid cell mapping
- nanoparticles
- SERRS
- pharmacology
- biomedical sciences
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Dive into the research topics of 'Rapid cell mapping using nanoparticles and SERRS'. Together they form a unique fingerprint.Projects
- 2 Finished
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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, D. (Principal Investigator), Dawson, M. (Co-investigator), Faulds, K. (Co-investigator), Girkin, J. (Co-investigator), Graham, D. (Co-investigator), Martin, R. (Co-investigator), O'Donnell, K. (Co-investigator), Rolinski, O. (Co-investigator), Smith, W. (Co-investigator) & Wynne, K. (Co-investigator)
Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)
1/08/08 → 31/01/12
Project: Research
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OPTICAL SPECTROSCOPIES FOR IMMUNO-BIOMEDICAL APPLICATIONS
Graham, D. (Principal Investigator), Alexander, J. (Co-investigator), Birch, D. (Co-investigator), Faulds, K. (Co-investigator), Garside, P. (Co-investigator) & Smith, W. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/08/06 → 31/01/12
Project: Research