Rapid cell mapping using nanoparticles and SERRS

Robert J. Stokes, Fiona McKenzie, Emma McFarlane, Alastair Ricketts, L. Tetley, Karen Faulds, J. Alexander, Duncan Graham

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
LanguageEnglish
Pages170-175
Number of pages6
JournalAnalyst
Volume134
Issue number1
DOIs
Publication statusPublished - 2009

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Raman Spectrum Analysis
Nanoparticles
Raman scattering
Coloring Agents
scattering
wavelength
Wavelength
Macrophages
Silver
Gold
fixation
bone
silver
dye
Bone
fluorescence
Dyes
gold
Lysosomes
Fluorescence

Keywords

  • rapid cell mapping
  • nanoparticles
  • SERRS
  • pharmacology
  • biomedical sciences

Cite this

Stokes, R. J., McKenzie, F., McFarlane, E., Ricketts, A., Tetley, L., Faulds, K., ... Graham, D. (2009). Rapid cell mapping using nanoparticles and SERRS. Analyst, 134(1), 170-175. https://doi.org/10.1039/b815117b
Stokes, Robert J. ; McKenzie, Fiona ; McFarlane, Emma ; Ricketts, Alastair ; Tetley, L. ; Faulds, Karen ; Alexander, J. ; Graham, Duncan. / Rapid cell mapping using nanoparticles and SERRS. In: Analyst. 2009 ; Vol. 134, No. 1. pp. 170-175.
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Stokes, RJ, McKenzie, F, McFarlane, E, Ricketts, A, Tetley, L, Faulds, K, Alexander, J & Graham, D 2009, 'Rapid cell mapping using nanoparticles and SERRS' Analyst, vol. 134, no. 1, pp. 170-175. https://doi.org/10.1039/b815117b

Rapid cell mapping using nanoparticles and SERRS. / Stokes, Robert J.; McKenzie, Fiona; McFarlane, Emma; Ricketts, Alastair; Tetley, L.; Faulds, Karen; Alexander, J.; Graham, Duncan.

In: Analyst, Vol. 134, No. 1, 2009, p. 170-175.

Research output: Contribution to journalArticle

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T1 - Rapid cell mapping using nanoparticles and SERRS

AU - Stokes, Robert J.

AU - McKenzie, Fiona

AU - McFarlane, Emma

AU - Ricketts, Alastair

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AU - Faulds, Karen

AU - Alexander, J.

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AB - Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.

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KW - SERRS

KW - pharmacology

KW - biomedical sciences

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Stokes RJ, McKenzie F, McFarlane E, Ricketts A, Tetley L, Faulds K et al. Rapid cell mapping using nanoparticles and SERRS. Analyst. 2009;134(1):170-175. https://doi.org/10.1039/b815117b