Rapid antibiotic susceptibility testing using low-cost, commercially available screen-printed electrodes

Research output: Contribution to journalArticle

Abstract

Antimicrobial resistance (AMR) is an issue of upmost global importance, with an annually increasing mortality rate and growing economic burden. Poor antimicrobial stewardship has resulted in an abundance and diverse range of antimicrobial resistance mechanisms. To tackle AMR effectively, better diagnostic tests must be developed in order to improve antibiotic stewardship and reduce the emergence of antibiotic resistant organisms. This study employs a low-cost, commercially available screen printed electrode modified with an agarose-based hydrogel deposit to monitor bacterial growth using the techniques of electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) giving rise to a new approach to measuring susceptibility. Susceptible and drug resistant Staphylococcus aureus strains were deposited onto agarose gel modified electrodes which contained clinically important antibiotics to establish growth profiles for each bacterial strain and monitor the influence of the antibiotic on bacterial growth. The results show that S. aureus is able to grow on electrodes modified with gel containing no antibiotic, but is inhibited when the gel modified electrode is seeded with antibiotic. Conversely, methicillin-resistant S. aureus (MRSA; the drug resistant strain) is able to grow on gel modified electrodes containing clinically relevant concentrations of antibiotic. Results show rapid growth profiles, with possible time to results for antibiotic susceptibility < 45 minutes, a significant improvement on the current gold standards of at least 1-2 days.
LanguageEnglish
Article number111696
Number of pages8
JournalBiosensors and Bioelectronics
Volume145
Early online date17 Sep 2019
DOIs
Publication statusE-pub ahead of print - 17 Sep 2019

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Antibiotics
Electrodes
Anti-Bacterial Agents
Costs and Cost Analysis
Testing
Costs
Gels
Methicillin-Resistant Staphylococcus aureus
Growth
Sepharose
Staphylococcus aureus
Dielectric Spectroscopy
Methicillin
Hydrogel
Voltammetry
Electrochemical impedance spectroscopy
Routine Diagnostic Tests
Hydrogels
Pharmaceutical Preparations
Deposits

Keywords

  • electrochemical biosensors
  • antibiotic susceptibility
  • growth profiles
  • electrochemical impedance spectroscopy (EIS)
  • antimicrobial resistance

Cite this

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title = "Rapid antibiotic susceptibility testing using low-cost, commercially available screen-printed electrodes",
abstract = "Antimicrobial resistance (AMR) is an issue of upmost global importance, with an annually increasing mortality rate and growing economic burden. Poor antimicrobial stewardship has resulted in an abundance and diverse range of antimicrobial resistance mechanisms. To tackle AMR effectively, better diagnostic tests must be developed in order to improve antibiotic stewardship and reduce the emergence of antibiotic resistant organisms. This study employs a low-cost, commercially available screen printed electrode modified with an agarose-based hydrogel deposit to monitor bacterial growth using the techniques of electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) giving rise to a new approach to measuring susceptibility. Susceptible and drug resistant Staphylococcus aureus strains were deposited onto agarose gel modified electrodes which contained clinically important antibiotics to establish growth profiles for each bacterial strain and monitor the influence of the antibiotic on bacterial growth. The results show that S. aureus is able to grow on electrodes modified with gel containing no antibiotic, but is inhibited when the gel modified electrode is seeded with antibiotic. Conversely, methicillin-resistant S. aureus (MRSA; the drug resistant strain) is able to grow on gel modified electrodes containing clinically relevant concentrations of antibiotic. Results show rapid growth profiles, with possible time to results for antibiotic susceptibility < 45 minutes, a significant improvement on the current gold standards of at least 1-2 days.",
keywords = "electrochemical biosensors, antibiotic susceptibility, growth profiles, electrochemical impedance spectroscopy (EIS), antimicrobial resistance",
author = "Stuart Hannah and Emily Addington and David Alcorn and Wenmiao Shu and Hoskisson, {Paul A.} and Corrigan, {Damion K.}",
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AB - Antimicrobial resistance (AMR) is an issue of upmost global importance, with an annually increasing mortality rate and growing economic burden. Poor antimicrobial stewardship has resulted in an abundance and diverse range of antimicrobial resistance mechanisms. To tackle AMR effectively, better diagnostic tests must be developed in order to improve antibiotic stewardship and reduce the emergence of antibiotic resistant organisms. This study employs a low-cost, commercially available screen printed electrode modified with an agarose-based hydrogel deposit to monitor bacterial growth using the techniques of electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) giving rise to a new approach to measuring susceptibility. Susceptible and drug resistant Staphylococcus aureus strains were deposited onto agarose gel modified electrodes which contained clinically important antibiotics to establish growth profiles for each bacterial strain and monitor the influence of the antibiotic on bacterial growth. The results show that S. aureus is able to grow on electrodes modified with gel containing no antibiotic, but is inhibited when the gel modified electrode is seeded with antibiotic. Conversely, methicillin-resistant S. aureus (MRSA; the drug resistant strain) is able to grow on gel modified electrodes containing clinically relevant concentrations of antibiotic. Results show rapid growth profiles, with possible time to results for antibiotic susceptibility < 45 minutes, a significant improvement on the current gold standards of at least 1-2 days.

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