Raman microscopy reveals how cell inflammation activates glucose and lipid metabolism

Aleksandra Borek-Dorosz, Anna Pieczara, Jagoda Orleanska, Krzysztof Brzozowski, William Tipping, Duncan Graham, Ewelina Bik, Adam Kubrak, Malgorzata Baranska, Katarzyna Majzner

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
26 Downloads (Pure)

Abstract

Metabolism of endothelial cells (ECs) depends on the availability of the energy substrates. Since the endothelium is the first line of defence against inflammation in the cardiovascular system and its dysfunction can lead to the development of cardiovascular diseases, it is important to understand how glucose metabolism changes during inflammation. In this work, glucose uptake was studied in human microvascular endothelial cells (HMEC-1) in high glucose (HG), and additionally in an inflammatory state, using Raman imaging. HG state was induced by incubation of ECs with a deuterated glucose analogue, while the EC inflammation was caused by TNF-α pre-treatment. Spontaneous and stimulated Raman scattering spectroscopy provided comprehensive information on biochemical changes, including lipids and the extent of unsaturation induced by excess glucose in ECs., induced by excess glucose in ECs. In this work, we indicated spectroscopic markers of metabolic changes in ECs as a strong increase in the ratio of the intensity of lipids / (proteins + lipids) bands and an increase in the level of lipid unsaturation and mitochondrial changes. Inflamed ECs treated with HG, revealed enhanced glucose uptake, and intensified lipid production i.a. of unsaturated lipids. Additionally, increased cytochrome c signal in the mitochondrial region indicated higher mitochondrial activity and biogenesis. Raman spectroscopy is a powerful method for determining the metabolic markers of ED which will better inform understanding of disease onset, development, and treatment.

Original languageEnglish
Article number119575
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1871
Issue number1
Early online date30 Sept 2023
DOIs
Publication statusPublished - 31 Jan 2024

Keywords

  • inflammation
  • cellular metabolism
  • Raman spectroscopy
  • coherent spectroscopy
  • endothelial cells
  • high glucose

Fingerprint

Dive into the research topics of 'Raman microscopy reveals how cell inflammation activates glucose and lipid metabolism'. Together they form a unique fingerprint.

Cite this