Quantum cascade laser-based mid-infrared spectrochemical imaging of tissue and biofluids

Graeme Clemens, Benjamin Bird, Miles Weida, Jeremy Rowlette, Matthew J. Baker

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mid-infrared spectroscopic imaging is a rapidly emerging technique in biomedical research and clinical diagnostics that takes advantage of the unique molecular fingerprint of cells, tissue and biofluids to provide a rich biochemical image without the need for staining. Spectroscopic analysis allows for the objective classification of biological material at a molecular level.1 This “label free” molecular imaging technique has been applied to histology, cytology, surgical pathology, microbiology and stem cell research, and can be used to detect subtle changes to the genome, proteome and metabolome.2,3,4 The new wealth of biochemical information made available by this technique has the distinct potential to improve cancer patient outcome through the identification of earlier stages of disease, drug resistance, new disease states and high-risk populations.4 However, despite the maturity of this science, instrumentation that provide increased sample throughput, improved image quality, a small footprint, low maintenance and require minimal spectral expertise are essential for clinical translation.
LanguageEnglish
Pages14-19
Number of pages6
JournalSpectroscopy Europe
Volume26
Issue number4
Publication statusPublished - 6 Aug 2014

Fingerprint

Quantum cascade lasers
Infrared imaging
quantum cascade lasers
Cytology
Tissue
Molecular imaging
Imaging techniques
Microbiology
Histology
Spectroscopic analysis
Pathology
Proteome
Stem cells
cells
proteome
microbiology
cytology
Biological materials
Image quality
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Keywords

  • spectrochemical imaging
  • biofluid diagnostics
  • tissue imaging
  • molecular imaging
  • Fourier transform infrared
  • FT-IR

Cite this

Clemens, Graeme ; Bird, Benjamin ; Weida, Miles ; Rowlette, Jeremy ; Baker, Matthew J. / Quantum cascade laser-based mid-infrared spectrochemical imaging of tissue and biofluids. In: Spectroscopy Europe. 2014 ; Vol. 26, No. 4. pp. 14-19.
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abstract = "Mid-infrared spectroscopic imaging is a rapidly emerging technique in biomedical research and clinical diagnostics that takes advantage of the unique molecular fingerprint of cells, tissue and biofluids to provide a rich biochemical image without the need for staining. Spectroscopic analysis allows for the objective classification of biological material at a molecular level.1 This “label free” molecular imaging technique has been applied to histology, cytology, surgical pathology, microbiology and stem cell research, and can be used to detect subtle changes to the genome, proteome and metabolome.2,3,4 The new wealth of biochemical information made available by this technique has the distinct potential to improve cancer patient outcome through the identification of earlier stages of disease, drug resistance, new disease states and high-risk populations.4 However, despite the maturity of this science, instrumentation that provide increased sample throughput, improved image quality, a small footprint, low maintenance and require minimal spectral expertise are essential for clinical translation.",
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Clemens, G, Bird, B, Weida, M, Rowlette, J & Baker, MJ 2014, 'Quantum cascade laser-based mid-infrared spectrochemical imaging of tissue and biofluids' Spectroscopy Europe, vol. 26, no. 4, pp. 14-19.

Quantum cascade laser-based mid-infrared spectrochemical imaging of tissue and biofluids. / Clemens, Graeme; Bird, Benjamin; Weida, Miles; Rowlette, Jeremy; Baker, Matthew J.

In: Spectroscopy Europe, Vol. 26, No. 4, 06.08.2014, p. 14-19.

Research output: Contribution to journalArticle

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