Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides

A. Macaskill; A.A Chernonosov; V.V Koval; E.A. Lukyanets; O.S. Fedorova; W.E. Smith; K. Faulds; D. Graham

doi:10.1093/nar/gkm042

Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides

A. Macaskill, A.A Chernonosov, V.V Koval, E.A. Lukyanets, O.S. Fedorova, W.E. Smith, K. Faulds, D. Graham

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.

Original language	English
Pages (from-to)	E42
Number of pages	6
Journal	Nucleic Acids Research
Volume	35
Issue number	6
DOIs	https://doi.org/10.1093/nar/gkm042
Publication status	Published - Mar 2007

Keywords

surface-enhanced resonance raman scattering
SERRS
phthalocyanine-labelled oligonucleotides

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title = "Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides",

abstract = "The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.",

keywords = "surface-enhanced resonance raman scattering, SERRS, phthalocyanine-labelled oligonucleotides",

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T1 - Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides

AU - Macaskill, A.

AU - Chernonosov, A.A

AU - Koval, V.V

AU - Lukyanets, E.A.

AU - Fedorova, O.S.

AU - Smith, W.E.

AU - Faulds, K.

AU - Graham, D.

PY - 2007/3

Y1 - 2007/3

N2 - The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.

AB - The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.

KW - surface-enhanced resonance raman scattering

KW - SERRS

KW - phthalocyanine-labelled oligonucleotides

U2 - 10.1093/nar/gkm042

DO - 10.1093/nar/gkm042

M3 - Article

VL - 35

SP - E42

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 6

ER -

Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides

Abstract

Keywords

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