Abstract
The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
Original language | English |
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Pages (from-to) | E42 |
Number of pages | 6 |
Journal | Nucleic Acids Research |
Volume | 35 |
Issue number | 6 |
DOIs | |
Publication status | Published - Mar 2007 |
Keywords
- surface-enhanced resonance raman scattering
- SERRS
- phthalocyanine-labelled oligonucleotides