Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides

A. Macaskill, A.A Chernonosov, V.V Koval, E.A. Lukyanets, O.S. Fedorova, W.E. Smith, K. Faulds, D. Graham

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
LanguageEnglish
PagesE42
Number of pages6
JournalNucleic Acids Research
Volume35
Issue number6
DOIs
Publication statusPublished - Mar 2007

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Raman Spectrum Analysis
Oligonucleotides
Limit of Detection
Silver
Nanoparticles
Metals
phthalocyanine
DNA

Keywords

  • surface-enhanced resonance raman scattering
  • SERRS
  • phthalocyanine-labelled oligonucleotides

Cite this

Macaskill, A. ; Chernonosov, A.A ; Koval, V.V ; Lukyanets, E.A. ; Fedorova, O.S. ; Smith, W.E. ; Faulds, K. ; Graham, D. / Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides. In: Nucleic Acids Research. 2007 ; Vol. 35, No. 6. pp. E42.
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Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides. / Macaskill, A.; Chernonosov, A.A; Koval, V.V; Lukyanets, E.A.; Fedorova, O.S.; Smith, W.E.; Faulds, K.; Graham, D.

In: Nucleic Acids Research, Vol. 35, No. 6, 03.2007, p. E42.

Research output: Contribution to journalArticle

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AU - Macaskill, A.

AU - Chernonosov, A.A

AU - Koval, V.V

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