Abstract
| Language | English |
|---|---|
| Pages | E42 |
| Number of pages | 6 |
| Journal | Nucleic Acids Research |
| Volume | 35 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Mar 2007 |
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Keywords
- surface-enhanced resonance raman scattering
- SERRS
- phthalocyanine-labelled oligonucleotides
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Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides. / Macaskill, A.; Chernonosov, A.A; Koval, V.V; Lukyanets, E.A.; Fedorova, O.S.; Smith, W.E.; Faulds, K.; Graham, D.
In: Nucleic Acids Research, Vol. 35, No. 6, 03.2007, p. E42.Research output: Contribution to journal › Article
TY - JOUR
T1 - Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides
AU - Macaskill, A.
AU - Chernonosov, A.A
AU - Koval, V.V
AU - Lukyanets, E.A.
AU - Fedorova, O.S.
AU - Smith, W.E.
AU - Faulds, K.
AU - Graham, D.
PY - 2007/3
Y1 - 2007/3
N2 - The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
AB - The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
KW - surface-enhanced resonance raman scattering
KW - SERRS
KW - phthalocyanine-labelled oligonucleotides
U2 - 10.1093/nar/gkm042
DO - 10.1093/nar/gkm042
M3 - Article
VL - 35
SP - E42
JO - Nucleic Acids Research
T2 - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 6
ER -