Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides

A. Macaskill, A.A Chernonosov, V.V Koval, E.A. Lukyanets, O.S. Fedorova, W.E. Smith, K. Faulds, D. Graham

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16 Citations (Scopus)


The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
Original languageEnglish
Pages (from-to)E42
Number of pages6
JournalNucleic Acids Research
Issue number6
Publication statusPublished - Mar 2007


  • surface-enhanced resonance raman scattering
  • phthalocyanine-labelled oligonucleotides


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