Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states

Nadine Ehmann, Sebastian van de Linde, Amit Alon, Dmitrij Ljaschenko, Xi Zhen Keung, Thorge Holm, Annika Rings, Aaron DiAntonio, Stefan Hallermann, Uri Ashery, Manfred Heckmann, Markus Sauer, Robert J. Kittel

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

The precise molecular architecture of synaptic active zones (AZs) gives rise to different structural and functional AZ states that fundamentally shape chemical neurotransmission. However, elucidating the nanoscopic protein arrangement at AZs is impeded by the diffraction-limited resolution of conventional light microscopy. Here we introduce new approaches to quantify endogenous protein organization at single-molecule resolution in situ with super-resolution imaging by direct stochastic optical reconstruction microscopy (dSTORM). Focusing on the Drosophila neuromuscular junction (NMJ), we find that the AZ cytomatrix (CAZ) is composed of units containing ~137 Bruchpilot (Brp) proteins, three quarters of which are organized into about 15 heptameric clusters. We test for a quantitative relationship between CAZ ultrastructure and neurotransmitter release properties by engaging Drosophila mutants and electrophysiology. Our results indicate that the precise nanoscopic organization of Brp distinguishes different physiological AZ states and link functional diversification to a heretofore unrecognized neuronal gradient of the CAZ ultrastructure.

LanguageEnglish
Article number4650
Number of pages12
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 18 Aug 2014

Fingerprint

Drosophila
proteins
Imaging techniques
Microscopy
electrophysiology
Electrophysiology
microscopy
neurotransmitters
Proteins
Neuromuscular Junction
Synaptic Transmission
Optical microscopy
Neurotransmitter Agents
Microscopic examination
Diffraction
Light
gradients
Molecules
diffraction
molecules

Keywords

  • synaptic active zones
  • direct stochastic optical reconstruction microscopy
  • neuromuscular junction
  • bruchpilot proteins
  • caz ultrastructure
  • az states
  • neurotransmitter release
  • Drosophila mutants
  • electrophysiology

Cite this

Ehmann, Nadine ; van de Linde, Sebastian ; Alon, Amit ; Ljaschenko, Dmitrij ; Keung, Xi Zhen ; Holm, Thorge ; Rings, Annika ; DiAntonio, Aaron ; Hallermann, Stefan ; Ashery, Uri ; Heckmann, Manfred ; Sauer, Markus ; Kittel, Robert J. / Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states. In: Nature Communications. 2014 ; Vol. 5.
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year = "2014",
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Ehmann, N, van de Linde, S, Alon, A, Ljaschenko, D, Keung, XZ, Holm, T, Rings, A, DiAntonio, A, Hallermann, S, Ashery, U, Heckmann, M, Sauer, M & Kittel, RJ 2014, 'Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states' Nature Communications, vol. 5, 4650. https://doi.org/10.1038/ncomms5650

Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states. / Ehmann, Nadine; van de Linde, Sebastian; Alon, Amit; Ljaschenko, Dmitrij; Keung, Xi Zhen; Holm, Thorge; Rings, Annika; DiAntonio, Aaron; Hallermann, Stefan; Ashery, Uri; Heckmann, Manfred; Sauer, Markus; Kittel, Robert J.

In: Nature Communications, Vol. 5, 4650, 18.08.2014.

Research output: Contribution to journalArticle

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AU - Holm, Thorge

AU - Rings, Annika

AU - DiAntonio, Aaron

AU - Hallermann, Stefan

AU - Ashery, Uri

AU - Heckmann, Manfred

AU - Sauer, Markus

AU - Kittel, Robert J.

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