Quantitative SERRS for DNA sequence analysis

Research output: Contribution to journalLiterature review

113 Citations (Scopus)

Abstract

SERRS is an extremely sensitive and selective technique which when applied to the detection of labelled DNA sequences allows detection limits to be obtained which rival, and in most cases are better than, fluorescence. In this tutorial review the conditions are explored which enable the successful detection of DNA using SERRS. The enhancing surface which is used is crucial and in this case suspensions of nanoparticles were the focus as they allow quantitative behaviour to be achieved in systems analogous to current fluorescence based approaches. The aggregation conditions required to obtain SERRS of DNA affect the sensitivity and the reproducibility and we describe the use of spermine as an effective aggregating agent to achieve excellent reproducibility and sensitivity. The nature of the label which is used, be it fluorescent or non fluorescent, positively or negatively charged, also affects the SERRS response and these conditions are again discussed. Finally, we show how to detect a specific target DNA sequence in a meaningful diagnostic assay using SERRS and how the approaches described previously in the review are vital to the success of such approaches.

LanguageEnglish
Pages1042-1051
Number of pages10
JournalChemical Society Reviews
Volume37
Issue number5
Early online date31 Mar 2008
DOIs
Publication statusPublished - 2008

Fingerprint

DNA sequences
Fluorescence
Spermine
DNA
Labels
Assays
Suspensions
Agglomeration
Nanoparticles

Keywords

  • RESONANCE RAMAN-SCATTERING
  • SURFACE-ENHANCED RAMAN
  • BENZOTRIAZOLE AZO-DYE
  • ON-A-CHIP
  • LABELED DNA
  • SILVER NANOPARTICLES
  • INFECTIOUS-DISEASES
  • GOLD
  • OLIGONUCLEOTIDES
  • FLUORESCENCE

Cite this

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title = "Quantitative SERRS for DNA sequence analysis",
abstract = "SERRS is an extremely sensitive and selective technique which when applied to the detection of labelled DNA sequences allows detection limits to be obtained which rival, and in most cases are better than, fluorescence. In this tutorial review the conditions are explored which enable the successful detection of DNA using SERRS. The enhancing surface which is used is crucial and in this case suspensions of nanoparticles were the focus as they allow quantitative behaviour to be achieved in systems analogous to current fluorescence based approaches. The aggregation conditions required to obtain SERRS of DNA affect the sensitivity and the reproducibility and we describe the use of spermine as an effective aggregating agent to achieve excellent reproducibility and sensitivity. The nature of the label which is used, be it fluorescent or non fluorescent, positively or negatively charged, also affects the SERRS response and these conditions are again discussed. Finally, we show how to detect a specific target DNA sequence in a meaningful diagnostic assay using SERRS and how the approaches described previously in the review are vital to the success of such approaches.",
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Quantitative SERRS for DNA sequence analysis. / Graham, Duncan; Faulds, Karen.

In: Chemical Society Reviews, Vol. 37, No. 5, 2008, p. 1042-1051.

Research output: Contribution to journalLiterature review

TY - JOUR

T1 - Quantitative SERRS for DNA sequence analysis

AU - Graham, Duncan

AU - Faulds, Karen

PY - 2008

Y1 - 2008

N2 - SERRS is an extremely sensitive and selective technique which when applied to the detection of labelled DNA sequences allows detection limits to be obtained which rival, and in most cases are better than, fluorescence. In this tutorial review the conditions are explored which enable the successful detection of DNA using SERRS. The enhancing surface which is used is crucial and in this case suspensions of nanoparticles were the focus as they allow quantitative behaviour to be achieved in systems analogous to current fluorescence based approaches. The aggregation conditions required to obtain SERRS of DNA affect the sensitivity and the reproducibility and we describe the use of spermine as an effective aggregating agent to achieve excellent reproducibility and sensitivity. The nature of the label which is used, be it fluorescent or non fluorescent, positively or negatively charged, also affects the SERRS response and these conditions are again discussed. Finally, we show how to detect a specific target DNA sequence in a meaningful diagnostic assay using SERRS and how the approaches described previously in the review are vital to the success of such approaches.

AB - SERRS is an extremely sensitive and selective technique which when applied to the detection of labelled DNA sequences allows detection limits to be obtained which rival, and in most cases are better than, fluorescence. In this tutorial review the conditions are explored which enable the successful detection of DNA using SERRS. The enhancing surface which is used is crucial and in this case suspensions of nanoparticles were the focus as they allow quantitative behaviour to be achieved in systems analogous to current fluorescence based approaches. The aggregation conditions required to obtain SERRS of DNA affect the sensitivity and the reproducibility and we describe the use of spermine as an effective aggregating agent to achieve excellent reproducibility and sensitivity. The nature of the label which is used, be it fluorescent or non fluorescent, positively or negatively charged, also affects the SERRS response and these conditions are again discussed. Finally, we show how to detect a specific target DNA sequence in a meaningful diagnostic assay using SERRS and how the approaches described previously in the review are vital to the success of such approaches.

KW - RESONANCE RAMAN-SCATTERING

KW - SURFACE-ENHANCED RAMAN

KW - BENZOTRIAZOLE AZO-DYE

KW - ON-A-CHIP

KW - LABELED DNA

KW - SILVER NANOPARTICLES

KW - INFECTIOUS-DISEASES

KW - GOLD

KW - OLIGONUCLEOTIDES

KW - FLUORESCENCE

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DO - 10.1039/b707941a

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JO - Chemical Society Reviews

T2 - Chemical Society Reviews

JF - Chemical Society Reviews

SN - 0306-0012

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