Quantitative experimental study of shear stress and mixing in progressive flow regimes within annular flow bioreactors

S.J. Curran, R.A. Black

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Annular-flow bioreactors are normally operated under laminar Couette flow conditions in order to minimise shear-induced damage to cells. In this study, we computed the fluid shear stresses in model annular vessels over a range of laminar flow regimes, from Couette flow to Taylor-vortex flow, and at two geometric scales, using a shear rate model for freely suspended particles, together with experimental Laser Doppler Anemometry data for a 2-D velocity field. The shear stresses were greatest in the boundary layers adjacent to each wall in each case, with values typically 6 times higher than the mean stresses in the annular space; their respective magnitudes were significantly lower in the larger of the two vessels studied, however. Cell viability studies were also performed in which mammalian cells were cultured under dynamic conditions in a functional bioreactor having the same dimensions as the smaller vessel. The results of these studies demonstrated that a significantly greater number of cells remained in suspension in Taylor-vortex flows than in Couette flow, but at the expense of cell viability at higher Taylor numbers. Taken together, these findings suggest that the benefits of enhanced convective mass transport afforded by Taylor-Couette flows could be realised without risk of appreciable shear induced damage of cells and tissues in larger vessels operating under dynamically similar flow conditions.
    LanguageEnglish
    Pages5859-5868
    Number of pages9
    JournalChemical Engineering Science
    Volume59
    Issue number24
    DOIs
    Publication statusPublished - 2004

    Fingerprint

    Bioreactor
    Bioreactors
    Shear Stress
    Shear stress
    Experimental Study
    Cells
    Laminar flow
    Vessel
    Couette Flow
    Cell
    Vortex flow
    Vortex Flow
    Laminar Flow
    Viability
    Shear deformation
    Suspensions
    Boundary layers
    Mass transfer
    Damage
    Tissue

    Keywords

    • hydrodynamics
    • mixing
    • transport processes
    • scale-up
    • molecular biology
    • sedimentation
    • bioengineering

    Cite this

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    abstract = "Annular-flow bioreactors are normally operated under laminar Couette flow conditions in order to minimise shear-induced damage to cells. In this study, we computed the fluid shear stresses in model annular vessels over a range of laminar flow regimes, from Couette flow to Taylor-vortex flow, and at two geometric scales, using a shear rate model for freely suspended particles, together with experimental Laser Doppler Anemometry data for a 2-D velocity field. The shear stresses were greatest in the boundary layers adjacent to each wall in each case, with values typically 6 times higher than the mean stresses in the annular space; their respective magnitudes were significantly lower in the larger of the two vessels studied, however. Cell viability studies were also performed in which mammalian cells were cultured under dynamic conditions in a functional bioreactor having the same dimensions as the smaller vessel. The results of these studies demonstrated that a significantly greater number of cells remained in suspension in Taylor-vortex flows than in Couette flow, but at the expense of cell viability at higher Taylor numbers. Taken together, these findings suggest that the benefits of enhanced convective mass transport afforded by Taylor-Couette flows could be realised without risk of appreciable shear induced damage of cells and tissues in larger vessels operating under dynamically similar flow conditions.",
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    Quantitative experimental study of shear stress and mixing in progressive flow regimes within annular flow bioreactors. / Curran, S.J.; Black, R.A.

    In: Chemical Engineering Science, Vol. 59, No. 24, 2004, p. 5859-5868.

    Research output: Contribution to journalArticle

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    AU - Black, R.A.

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