Quantitative enhanced Raman scattering of labelled DNA from gold and silver nanoparticles

Robert J. Stokes, A. Macaskill, P.J. Lundahl, W.E. Smith, K. Faulds, D. Graham

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Surface-enhanced resonance Raman scattering (SERRS) from silver nanoparticles using 514.5-nm excitation has been shown to offer huge potential for applications in highly sensitive multiplexed DNA assays. If the technique is to be applied to real biological samples and integrated with other methods, then the use of gold nanoparticles and longer wavelengths of excitation are desirable. The data presented here demonstrate that dye-labeled oligonucleotide sequences can be directly detected by SERRS using gold nanoparticles in a quantitative manner for the first time. The performance of gold and silver nanoparticles as SERRS substrates was assessed using 514.5-, 632.8-, and 785-nm excitation and a range of 13 commercially available dye-labeled oligonucleotides. The quantitative response allowed the limit of detection to be determined for each case and demonstrates that the technique is highly effective, sensitive, and versatile. The possibility of excitation at multiple wavelengths further enhances the multiplexing potential of the technique. The importance of effectively combining the optical properties of the nanoparticle and the dye label is demonstrated. For example, at 632.8-nm excitation, the dye BODIPY TR-X and gold nanoparticles make a strong SERRS combination with very little background fluorescence. This study allows the choice of nanoparticle and dye label for particular experimental setups, and significantly expands the applicability of enhanced Raman scattering for use in many disciplines.
Original languageEnglish
Pages (from-to)1593-1601
Number of pages9
JournalSmall
Volume3
Issue number9
DOIs
Publication statusPublished - 3 Sep 2007

Keywords

  • DNA
  • dyes
  • nanoparticles
  • oligonucleotides
  • Raman spectroscopy
  • surface-enhanced resonance Raman scattering
  • SERRS

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    Multiple target molecular diagnostics testing provides better diagnostics for healthcare

    Duncan Graham (Participant), W. Ewen Smith (Participant) & Karen Faulds (Participant)

    Impact: Impact - for External PortalEconomic and commerce, Health and welfare - new products, guidelines and services

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