Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

F. McKenzie, V. Steven, A. Ingram, D. Graham

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.
LanguageEnglish
Pages2872-2874
Number of pages2
JournalChemical Communications (London)
Issue number20
DOIs
Publication statusPublished - 31 Mar 2009

Fingerprint

Biomolecules
Hydrolysis
Nanoparticles
Deoxyribonuclease I
Enzymes
Silver
Gold
Trypsin
Labels
Antibodies
DNA

Keywords

  • biomolecules
  • gold nanoparticles
  • silver nanoparticles
  • immobilised biomolecules
  • fluorescent-labelled antibodies
  • DNA

Cite this

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Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis. / McKenzie, F.; Steven, V.; Ingram, A.; Graham, D.

In: Chemical Communications (London), No. 20, 31.03.2009, p. 2872-2874.

Research output: Contribution to journalArticle

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KW - fluorescent-labelled antibodies

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