Abstract
| Language | English |
|---|---|
| Pages | 2872-2874 |
| Number of pages | 2 |
| Journal | Chemical Communications (London) |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 31 Mar 2009 |
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Keywords
- biomolecules
- gold nanoparticles
- silver nanoparticles
- immobilised biomolecules
- fluorescent-labelled antibodies
- DNA
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Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis. / McKenzie, F.; Steven, V.; Ingram, A.; Graham, D.
In: Chemical Communications (London), No. 20, 31.03.2009, p. 2872-2874.Research output: Contribution to journal › Article
TY - JOUR
T1 - Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis
AU - McKenzie, F.
AU - Steven, V.
AU - Ingram, A.
AU - Graham, D.
PY - 2009/3/31
Y1 - 2009/3/31
N2 - A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.
AB - A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.
KW - biomolecules
KW - gold nanoparticles
KW - silver nanoparticles
KW - immobilised biomolecules
KW - fluorescent-labelled antibodies
KW - DNA
UR - http://dx.doi.org/10.1039/b823057a
U2 - 10.1039/b823057a
DO - 10.1039/b823057a
M3 - Article
SP - 2872
EP - 2874
JO - Chemical Communications (London)
T2 - Chemical Communications (London)
JF - Chemical Communications (London)
SN - 0009-241X
IS - 20
ER -