Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites

Shakhawan K. Mawlood, Lynn Dennany, Nigel Watson, John Dempster, Benjamin S. Pickard

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mammalian ageing features biological attrition evident at cellular, genetic and epigenetic levels. Mutation of mitochondrial DNA, and nuclear DNA methylation changes are well established correlates of ageing. The methylation of mitochondrial DNA (mtDNA) is a new and incompletely described phenomenon with unknown biological control and significance. Here we describe the bisulphite sequencing of mtDNA from 82 individuals aged 18-91 years. We detected low and variable levels of mtDNA methylation at 54 of 133 CpG sites interrogated. Regression analysis of methylation levels at two CpG sites (M1215 and M1313) located within the 12S ribosomal RNA gene showed an inverse correlation with subject age suggesting their utility as epigenetic markers of ageing.
LanguageEnglish
Pages636-641
Number of pages6
JournalAging
Volume8
Issue number4
Publication statusPublished - 17 Feb 2016

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DNA Methylation
Mitochondrial DNA
Epigenomics
Methylation
rRNA Genes
Regression Analysis
Mutation

Keywords

  • mammalian ageing
  • mitochondrial DNA
  • aging markers

Cite this

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Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites. / Mawlood, Shakhawan K.; Dennany, Lynn; Watson, Nigel; Dempster, John; Pickard, Benjamin S.

In: Aging, Vol. 8, No. 4, 17.02.2016, p. 636-641.

Research output: Contribution to journalArticle

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T1 - Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites

AU - Mawlood, Shakhawan K.

AU - Dennany, Lynn

AU - Watson, Nigel

AU - Dempster, John

AU - Pickard, Benjamin S.

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AB - Mammalian ageing features biological attrition evident at cellular, genetic and epigenetic levels. Mutation of mitochondrial DNA, and nuclear DNA methylation changes are well established correlates of ageing. The methylation of mitochondrial DNA (mtDNA) is a new and incompletely described phenomenon with unknown biological control and significance. Here we describe the bisulphite sequencing of mtDNA from 82 individuals aged 18-91 years. We detected low and variable levels of mtDNA methylation at 54 of 133 CpG sites interrogated. Regression analysis of methylation levels at two CpG sites (M1215 and M1313) located within the 12S ribosomal RNA gene showed an inverse correlation with subject age suggesting their utility as epigenetic markers of ageing.

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KW - aging markers

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