Qualitative SERS analysis of G-quadruplex DNAs using selective stabilising ligands

K. Gracie, V. Dhamodharan, P. I. Pradeepkumar, K. Faulds, D. Graham

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nucleic acids are of key biological importance due to their range of functions and ability to form various different structures, with an example of emerging significance being quadruplexes formed by guanine-rich sequences. These guanine rich sequences are found in different regions of the genome such as telomeres, gene promoters and introns and UTRs of mRNAs. Here a new approach has been developed that utilises surface enhanced Raman scattering (SERS) for the detection of the formation of G-quadruplexes. Three G-quadruplex stabilising ligands that each have their own unique SERS response were used in this study and their ability to act as reporters assessed. A SERS response was only obtained from the ligands in the absence of G-quadruplex formation. This resulted in an "on/off" method which was successfully used to qualitatively detect the formation of G-quadruplex using quadruplex-forming sequences such as human telomeric and C-MYC promoter DNAs. The unique SERS spectra of each stabilising ligand offer the potential for use of SERS to study higher order DNA structures. This work shows that the ligands used can act simultaneously as a potential therapeutic stabilising agent and a SERS reporter, therefore allowing the use of SERS as a method of analysis of the formation of G-quadruplex DNAs.

LanguageEnglish
Pages4458-4465
Number of pages8
JournalAnalyst
Volume139
Issue number18
Early online date24 Apr 2014
DOIs
Publication statusPublished - 21 Sep 2014

Fingerprint

G-Quadruplexes
Raman Spectrum Analysis
ligand
Raman scattering
DNA
Ligands
scattering
Guanine
Genes
Untranslated Regions
Excipients
Nucleic acids
Telomere
nucleic acid
analysis
Introns
Nucleic Acids
genome
Genome
Messenger RNA

Keywords

  • selective stabilising ligands
  • qualitative SERS analysis
  • G-quadruplex DNAs

Cite this

Gracie, K. ; Dhamodharan, V. ; Pradeepkumar, P. I. ; Faulds, K. ; Graham, D. / Qualitative SERS analysis of G-quadruplex DNAs using selective stabilising ligands. In: Analyst. 2014 ; Vol. 139, No. 18. pp. 4458-4465.
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Qualitative SERS analysis of G-quadruplex DNAs using selective stabilising ligands. / Gracie, K.; Dhamodharan, V.; Pradeepkumar, P. I.; Faulds, K.; Graham, D.

In: Analyst, Vol. 139, No. 18, 21.09.2014, p. 4458-4465.

Research output: Contribution to journalArticle

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