Purification of a 5-ht uptake inhibitor from the venom of cerastes vipera

M. Alzahaby; A.L. Harvey; L.C. Young; G. Faure; E.G. Rowan

doi:10.1016/S0041-0101(97)00108-6

Purification of a 5-ht uptake inhibitor from the venom of cerastes vipera

M. Alzahaby, A.L. Harvey, L.C. Young, G. Faure, E.G. Rowan

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A protein that inhibits the re-uptake of 5-hydroxytryptamine into rat brain synaptosomes was isolated from the venom of the Sahara sand viper (Cerastes vipera) by gel filtration and reverse phase chromatography. It has a molecular weight of 13 739 Da and an 50 of about 50 nM for blocking uptake of 3H-5-HT into rat brain synaptosomes. It also augmented the responses to 5-HT in a smooth muscle preparation. It has phospholipase A2 activity, but it has no lytic activity as measured by its inability to release lactate dehydrogenase from rat brain synaptosomes. Determination of the N-terminal sequence revealed a similarity with a phospholipase A2 previously isolated from Cerastes cerastes venom.

Original language	English
Pages (from-to)	601-607
Number of pages	6
Journal	Toxicon
Volume	36
Issue number	4
DOIs	https://doi.org/10.1016/S0041-0101(97)00108-6
Publication status	Published - Apr 1998

Keywords

5-ht uptake inhibitor
venom
cerastes vipera

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10.1016/S0041-0101(97)00108-6

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title = "Purification of a 5-ht uptake inhibitor from the venom of cerastes vipera",

abstract = "A protein that inhibits the re-uptake of 5-hydroxytryptamine into rat brain synaptosomes was isolated from the venom of the Sahara sand viper (Cerastes vipera) by gel filtration and reverse phase chromatography. It has a molecular weight of 13 739 Da and an 50 of about 50 nM for blocking uptake of 3H-5-HT into rat brain synaptosomes. It also augmented the responses to 5-HT in a smooth muscle preparation. It has phospholipase A2 activity, but it has no lytic activity as measured by its inability to release lactate dehydrogenase from rat brain synaptosomes. Determination of the N-terminal sequence revealed a similarity with a phospholipase A2 previously isolated from Cerastes cerastes venom.",

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T1 - Purification of a 5-ht uptake inhibitor from the venom of cerastes vipera

AU - Alzahaby, M.

AU - Harvey, A.L.

AU - Young, L.C.

AU - Faure, G.

AU - Rowan, E.G.

PY - 1998/4

Y1 - 1998/4

N2 - A protein that inhibits the re-uptake of 5-hydroxytryptamine into rat brain synaptosomes was isolated from the venom of the Sahara sand viper (Cerastes vipera) by gel filtration and reverse phase chromatography. It has a molecular weight of 13 739 Da and an 50 of about 50 nM for blocking uptake of 3H-5-HT into rat brain synaptosomes. It also augmented the responses to 5-HT in a smooth muscle preparation. It has phospholipase A2 activity, but it has no lytic activity as measured by its inability to release lactate dehydrogenase from rat brain synaptosomes. Determination of the N-terminal sequence revealed a similarity with a phospholipase A2 previously isolated from Cerastes cerastes venom.

AB - A protein that inhibits the re-uptake of 5-hydroxytryptamine into rat brain synaptosomes was isolated from the venom of the Sahara sand viper (Cerastes vipera) by gel filtration and reverse phase chromatography. It has a molecular weight of 13 739 Da and an 50 of about 50 nM for blocking uptake of 3H-5-HT into rat brain synaptosomes. It also augmented the responses to 5-HT in a smooth muscle preparation. It has phospholipase A2 activity, but it has no lytic activity as measured by its inability to release lactate dehydrogenase from rat brain synaptosomes. Determination of the N-terminal sequence revealed a similarity with a phospholipase A2 previously isolated from Cerastes cerastes venom.

KW - 5-ht uptake inhibitor

KW - venom

KW - cerastes vipera

UR - http://dx.doi.org/10.1016/S0041-0101(97)00108-6

U2 - 10.1016/S0041-0101(97)00108-6

DO - 10.1016/S0041-0101(97)00108-6

M3 - Article

SN - 0041-0101

VL - 36

SP - 601

EP - 607

JO - Toxicon

JF - Toxicon

IS - 4

ER -

Purification of a 5-ht uptake inhibitor from the venom of cerastes vipera

Abstract

Keywords

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