Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms

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Pulsed electric field (PEF) treatment can be used for non-thermal inactivation of microorganisms. The aim of this paper is to investigate PEF treatment of yeast, Saccharomyces cerevisiae, using three different field waveforms: square; non-oscillating exponential and oscillating exponential. The PEF system used in this paper consists of a pulsed power supply and a parallel-plane metallic electrodes treatment cell located in an air-pressurised chamber. PEF treatment of the yeast was conducted using electric field impulses with magnitudes of 67 kV/cm and 80 kV/cm. The efficacy of the PEF treatment for inactivation of the yeast cells was assessed by comparison of the PEF-treated and untreated yeast populations. Results showed that 3-log10 reduction in the yeast population can be achieved with 100 impulses using all tested waveforms. Amongst all three tested waveforms non-oscillating exponential impulses demonstrated improved PEF performance. The effect of duration of treatment and peak magnitude of the field on the PEF process is discussed.
Original languageEnglish
Pages (from-to)1841-1848
Number of pages7
JournalIEEE Transactions on Dielectrics and Electrical Insulation
Issue number4
Publication statusPublished - 11 Aug 2015


  • pulsed electric field
  • inactivation
  • yeast
  • Saccharomyces cerevisiae
  • field waveforms
  • PEF systems


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