Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms

S. Qin; I. V. Timoshkin; M. MacLean; M. P. Wilson; M. J. Given; T. Wang; J. G. Anderson; S. J. MacGregor

doi:10.1109/TDEI.2015.005152

Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms

S. Qin, I. V. Timoshkin, M. MacLean, M. P. Wilson, M. J. Given, T. Wang, J. G. Anderson, S. J. MacGregor

Electronic And Electrical Engineering

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Abstract

Pulsed electric field (PEF) treatment can be used for non-thermal inactivation of microorganisms. The aim of this paper is to investigate PEF treatment of yeast, Saccharomyces cerevisiae, using three different field waveforms: square; non-oscillating exponential and oscillating exponential. The PEF system used in this paper consists of a pulsed power supply and a parallel-plane metallic electrodes treatment cell located in an air-pressurised chamber. PEF treatment of the yeast was conducted using electric field impulses with magnitudes of 67 kV/cm and 80 kV/cm. The efficacy of the PEF treatment for inactivation of the yeast cells was assessed by comparison of the PEF-treated and untreated yeast populations. Results showed that 3-log10 reduction in the yeast population can be achieved with 100 impulses using all tested waveforms. Amongst all three tested waveforms non-oscillating exponential impulses demonstrated improved PEF performance. The effect of duration of treatment and peak magnitude of the field on the PEF process is discussed.

Original language	English
Pages (from-to)	1841-1848
Number of pages	7
Journal	IEEE Transactions on Dielectrics and Electrical Insulation
Volume	22
Issue number	4
DOIs	https://doi.org/10.1109/TDEI.2015.005152
Publication status	Published - 11 Aug 2015

Keywords

pulsed electric field
inactivation
yeast
Saccharomyces cerevisiae
field waveforms
PEF systems

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Qin-etal-TDEI2015-pulsed-electric-field-treatment-of-saccharomyces-cerevisiaeAccepted author manuscript, 839 KB

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@article{a3dae83852b74b138a55c6024b0993fb,

title = "Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms",

abstract = "Pulsed electric field (PEF) treatment can be used for non-thermal inactivation of microorganisms. The aim of this paper is to investigate PEF treatment of yeast, Saccharomyces cerevisiae, using three different field waveforms: square; non-oscillating exponential and oscillating exponential. The PEF system used in this paper consists of a pulsed power supply and a parallel-plane metallic electrodes treatment cell located in an air-pressurised chamber. PEF treatment of the yeast was conducted using electric field impulses with magnitudes of 67 kV/cm and 80 kV/cm. The efficacy of the PEF treatment for inactivation of the yeast cells was assessed by comparison of the PEF-treated and untreated yeast populations. Results showed that 3-log10 reduction in the yeast population can be achieved with 100 impulses using all tested waveforms. Amongst all three tested waveforms non-oscillating exponential impulses demonstrated improved PEF performance. The effect of duration of treatment and peak magnitude of the field on the PEF process is discussed.",

keywords = "pulsed electric field, inactivation, yeast, Saccharomyces cerevisiae, field waveforms, PEF systems",

author = "S. Qin and Timoshkin, {I. V.} and M. MacLean and Wilson, {M. P.} and Given, {M. J.} and T. Wang and Anderson, {J. G.} and MacGregor, {S. J.}",

note = " {\textcopyright} 2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.",

year = "2015",

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doi = "10.1109/TDEI.2015.005152",

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T1 - Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms

AU - Qin, S.

AU - Timoshkin, I. V.

AU - MacLean, M.

AU - Wilson, M. P.

AU - Given, M. J.

AU - Wang, T.

AU - Anderson, J. G.

AU - MacGregor, S. J.

N1 - © 2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.

PY - 2015/8/11

Y1 - 2015/8/11

N2 - Pulsed electric field (PEF) treatment can be used for non-thermal inactivation of microorganisms. The aim of this paper is to investigate PEF treatment of yeast, Saccharomyces cerevisiae, using three different field waveforms: square; non-oscillating exponential and oscillating exponential. The PEF system used in this paper consists of a pulsed power supply and a parallel-plane metallic electrodes treatment cell located in an air-pressurised chamber. PEF treatment of the yeast was conducted using electric field impulses with magnitudes of 67 kV/cm and 80 kV/cm. The efficacy of the PEF treatment for inactivation of the yeast cells was assessed by comparison of the PEF-treated and untreated yeast populations. Results showed that 3-log10 reduction in the yeast population can be achieved with 100 impulses using all tested waveforms. Amongst all three tested waveforms non-oscillating exponential impulses demonstrated improved PEF performance. The effect of duration of treatment and peak magnitude of the field on the PEF process is discussed.

AB - Pulsed electric field (PEF) treatment can be used for non-thermal inactivation of microorganisms. The aim of this paper is to investigate PEF treatment of yeast, Saccharomyces cerevisiae, using three different field waveforms: square; non-oscillating exponential and oscillating exponential. The PEF system used in this paper consists of a pulsed power supply and a parallel-plane metallic electrodes treatment cell located in an air-pressurised chamber. PEF treatment of the yeast was conducted using electric field impulses with magnitudes of 67 kV/cm and 80 kV/cm. The efficacy of the PEF treatment for inactivation of the yeast cells was assessed by comparison of the PEF-treated and untreated yeast populations. Results showed that 3-log10 reduction in the yeast population can be achieved with 100 impulses using all tested waveforms. Amongst all three tested waveforms non-oscillating exponential impulses demonstrated improved PEF performance. The effect of duration of treatment and peak magnitude of the field on the PEF process is discussed.

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Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms

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Pulsed electric field treatment on Saccharomyces cerevisiae using different waveforms

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