Abstract
The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast extract agar and Bacillus cereus-selective agar plates. Following exposure to lethal levels of PEF, TEN investigation revealed irreversible cell membrane rupture at a number of locations, with the apparent leakage of intracellular contents. This study provides a clearer understanding of the mechanism of PEF-induced cellular damage, information that is essential for the further optimization of this emerging food-processing technology.
| Original language | English |
|---|---|
| Pages (from-to) | 110-114 |
| Number of pages | 5 |
| Journal | Letters in Applied Microbiology |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 31 Aug 2000 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- agar
- microscopy, electron
- Bacillus cereus
- controlled study
- electric field
- electroporation
- nonhuman
- transmission electron microscopy
- Bacillaceae infections
- colony count, microbial
- diarrhea
- electricity
- food handling
- humans
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