Pulsed electric field inactivation of diarrhoeagenic Bacillus cereus through irreversible electroporation

N.J. Rowan; S.J. MacGregor; J.G. Anderson; R.A. Fouracre; O. Farish

doi:10.1046/j.1365-2672.2000.00772.x

Pulsed electric field inactivation of diarrhoeagenic Bacillus cereus through irreversible electroporation

N.J. Rowan, S.J. MacGregor, J.G. Anderson, R.A. Fouracre, O. Farish

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Abstract

The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast extract agar and Bacillus cereus-selective agar plates. Following exposure to lethal levels of PEF, TEN investigation revealed irreversible cell membrane rupture at a number of locations, with the apparent leakage of intracellular contents. This study provides a clearer understanding of the mechanism of PEF-induced cellular damage, information that is essential for the further optimization of this emerging food-processing technology.

Original language	English
Pages (from-to)	110-114
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	31
Issue number	2
DOIs	https://doi.org/10.1046/j.1365-2672.2000.00772.x
Publication status	Published - 31 Aug 2000

Keywords

agar
microscopy, electron
Bacillus cereus
controlled study
electric field
electroporation
nonhuman
transmission electron microscopy
Bacillaceae infections
colony count, microbial
diarrhea
electricity
food handling
humans

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1046/j.1365-2672.2000.00772.x

Cite this

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title = "Pulsed electric field inactivation of diarrhoeagenic Bacillus cereus through irreversible electroporation",

abstract = "The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast extract agar and Bacillus cereus-selective agar plates. Following exposure to lethal levels of PEF, TEN investigation revealed irreversible cell membrane rupture at a number of locations, with the apparent leakage of intracellular contents. This study provides a clearer understanding of the mechanism of PEF-induced cellular damage, information that is essential for the further optimization of this emerging food-processing technology.",

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doi = "10.1046/j.1365-2672.2000.00772.x",

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T1 - Pulsed electric field inactivation of diarrhoeagenic Bacillus cereus through irreversible electroporation

AU - Rowan, N.J.

AU - MacGregor, S.J.

AU - Anderson, J.G.

AU - Fouracre, R.A.

AU - Farish, O.

PY - 2000/8/31

Y1 - 2000/8/31

N2 - The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast extract agar and Bacillus cereus-selective agar plates. Following exposure to lethal levels of PEF, TEN investigation revealed irreversible cell membrane rupture at a number of locations, with the apparent leakage of intracellular contents. This study provides a clearer understanding of the mechanism of PEF-induced cellular damage, information that is essential for the further optimization of this emerging food-processing technology.

AB - The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast extract agar and Bacillus cereus-selective agar plates. Following exposure to lethal levels of PEF, TEN investigation revealed irreversible cell membrane rupture at a number of locations, with the apparent leakage of intracellular contents. This study provides a clearer understanding of the mechanism of PEF-induced cellular damage, information that is essential for the further optimization of this emerging food-processing technology.

KW - agar

KW - microscopy, electron

KW - Bacillus cereus

KW - controlled study

KW - electric field

KW - electroporation

KW - nonhuman

KW - transmission electron microscopy

KW - Bacillaceae infections

KW - colony count, microbial

KW - diarrhea

KW - electricity

KW - food handling

KW - humans

U2 - 10.1046/j.1365-2672.2000.00772.x

DO - 10.1046/j.1365-2672.2000.00772.x

M3 - Article

SN - 0266-8254

VL - 31

SP - 110

EP - 114

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 2

ER -

Pulsed electric field inactivation of diarrhoeagenic Bacillus cereus through irreversible electroporation

Abstract

Keywords

UN SDGs

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