Pulse shapes for optimized two-photon laser scanning microscopy

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1 Citation (Scopus)

Abstract

A numerical model describing the effect of different pulse shapes on the probability of two-photon absorption for two-photon laser scanning microscopy (2PLSM) is reported. Despite the commercial preference for sech2 pulses, this pulse shape does not provide the best performance. The presented model reveals that a square or parabolic pulse shape is preferable and shows that applications of such pulse shapes can improve the probability of two-photon absorption by up to 50% to facilitate improved 2PLSM.
Original languageEnglish
Pages (from-to)205502-205502
Number of pages0
JournalJournal of Physics D: Applied Physics
Volume41
Issue number20
DOIs
Publication statusPublished - 21 Oct 2008

Keywords

  • photon laser scanning microscopy
  • (2PLSM)

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