Pulse shapes for optimized two-photon laser scanning microscopy

Research output: Contribution to journalArticle

Abstract

A numerical model describing the effect of different pulse shapes on the probability of two-photon absorption for two-photon laser scanning microscopy (2PLSM) is reported. Despite the commercial preference for sech2 pulses, this pulse shape does not provide the best performance. The presented model reveals that a square or parabolic pulse shape is preferable and shows that applications of such pulse shapes can improve the probability of two-photon absorption by up to 50% to facilitate improved 2PLSM.
LanguageEnglish
Pages205502-205502
Number of pages0
JournalJournal of Physics D: Applied Physics
Volume41
Issue number20
DOIs
Publication statusPublished - 21 Oct 2008

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Laser pulses
Microscopic examination
Photons
microscopy
Scanning
scanning
Lasers
photons
pulses
lasers
Numerical models

Keywords

  • photon laser scanning microscopy
  • (2PLSM)

Cite this

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title = "Pulse shapes for optimized two-photon laser scanning microscopy",
abstract = "A numerical model describing the effect of different pulse shapes on the probability of two-photon absorption for two-photon laser scanning microscopy (2PLSM) is reported. Despite the commercial preference for sech2 pulses, this pulse shape does not provide the best performance. The presented model reveals that a square or parabolic pulse shape is preferable and shows that applications of such pulse shapes can improve the probability of two-photon absorption by up to 50{\%} to facilitate improved 2PLSM.",
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Pulse shapes for optimized two-photon laser scanning microscopy. / McConnell, G.

In: Journal of Physics D: Applied Physics, Vol. 41, No. 20, 21.10.2008, p. 205502-205502.

Research output: Contribution to journalArticle

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