Proteomic analysis of Lactobacillus pentosus for the identification of potential markers of adhesion and other probiotic features

Beatriz Pérez Montoro, Nabil Benomar, Natacha Cabellero Gomez, Said Ennahar, Peter Horvatovich, Charles Knapp, Esther Alonso, Antonio Gálvez, Hikmate Abriouel

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Abstract

We analyzed the adhesion capacity to mucus of 31 Lactobacillus pentosus strains isolated from naturally fermented Aloreña green table olives using an immobilized mucin model. On the basis of their adhesive capacity to mucin, three phenotypes were selected for cell-wall protein proteomic analysis to pinpoint proteins involved in the adhesion process: the highly adhesive L. pentosus CF1-43 N (73.49% of adhesion ability), the moderately adhesive L. pentosus CF1-37 N (49.56% of adhesion ability) and the poorly adhesive L. pentosus CF2-20P (32.79% of adhesion ability). The results revealed four moonlighting proteins over-produced in the highly adhesive L. pentosus CF1-43 N, which were under/not produced in the other two L. pentosus strains (CF1-37 N and CF2-20P). These proteins were involved in glycolytic pathway (phosphoglycerate mutase and glucosamine-6-phosphate deaminase), stress response (small heat shock protein) and transcription (transcription elongation factor GreA). Furthermore, the relative fold change in gene expression analysis showed significant up-regulation of the genes coding for these four moonlighting proteins in the highly adhesive L. pentosus CF1-43 N versus the poorly adhesive L. pentosus CF2-20P and also in response to mucin for 20 h which clearly indicate the significant role of these genes in the adhesion capacity of L. pentosus. Thus, these proteins could be used as biomarkers for mucus adhesion in L. pentosus. On the other hand, mucin exposure induced other probiotic effects in L. pentosus strains, enhancing their co-aggregation ability with pathogens and possible inactivation.
Original languageEnglish
Pages (from-to)58-66
Number of pages9
JournalFood Research International
Volume111
Early online date9 May 2018
DOIs
Publication statusPublished - 1 Sep 2018

Fingerprint

Lactobacillus pentosus
Probiotics
Proteomics
proteomics
probiotics
adhesion
adhesives
Adhesives
mucins
Mucins
Proteins
proteins
Mucus
mucus
transcription (genetics)
Phosphoglycerate Mutase
Small Heat-Shock Proteins
Peptide Elongation Factors
phosphoglyceric acids
glucosamine

Keywords

  • Lactobacillus pentosus
  • proteomics
  • probiotics
  • mucus
  • adhesion
  • biomarkers
  • qRT-PCR

Cite this

Pérez Montoro, Beatriz ; Benomar, Nabil ; Gomez, Natacha Cabellero ; Ennahar, Said ; Horvatovich, Peter ; Knapp, Charles ; Alonso, Esther ; Gálvez, Antonio ; Abriouel, Hikmate. / Proteomic analysis of Lactobacillus pentosus for the identification of potential markers of adhesion and other probiotic features. In: Food Research International. 2018 ; Vol. 111. pp. 58-66.
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abstract = "We analyzed the adhesion capacity to mucus of 31 Lactobacillus pentosus strains isolated from naturally fermented Alore{\~n}a green table olives using an immobilized mucin model. On the basis of their adhesive capacity to mucin, three phenotypes were selected for cell-wall protein proteomic analysis to pinpoint proteins involved in the adhesion process: the highly adhesive L. pentosus CF1-43 N (73.49{\%} of adhesion ability), the moderately adhesive L. pentosus CF1-37 N (49.56{\%} of adhesion ability) and the poorly adhesive L. pentosus CF2-20P (32.79{\%} of adhesion ability). The results revealed four moonlighting proteins over-produced in the highly adhesive L. pentosus CF1-43 N, which were under/not produced in the other two L. pentosus strains (CF1-37 N and CF2-20P). These proteins were involved in glycolytic pathway (phosphoglycerate mutase and glucosamine-6-phosphate deaminase), stress response (small heat shock protein) and transcription (transcription elongation factor GreA). Furthermore, the relative fold change in gene expression analysis showed significant up-regulation of the genes coding for these four moonlighting proteins in the highly adhesive L. pentosus CF1-43 N versus the poorly adhesive L. pentosus CF2-20P and also in response to mucin for 20 h which clearly indicate the significant role of these genes in the adhesion capacity of L. pentosus. Thus, these proteins could be used as biomarkers for mucus adhesion in L. pentosus. On the other hand, mucin exposure induced other probiotic effects in L. pentosus strains, enhancing their co-aggregation ability with pathogens and possible inactivation.",
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Pérez Montoro, B, Benomar, N, Gomez, NC, Ennahar, S, Horvatovich, P, Knapp, C, Alonso, E, Gálvez, A & Abriouel, H 2018, 'Proteomic analysis of Lactobacillus pentosus for the identification of potential markers of adhesion and other probiotic features', Food Research International, vol. 111, pp. 58-66. https://doi.org/10.1016/j.foodres.2018.04.072

Proteomic analysis of Lactobacillus pentosus for the identification of potential markers of adhesion and other probiotic features. / Pérez Montoro, Beatriz; Benomar, Nabil; Gomez, Natacha Cabellero; Ennahar, Said; Horvatovich, Peter; Knapp, Charles; Alonso, Esther; Gálvez, Antonio; Abriouel, Hikmate.

In: Food Research International, Vol. 111, 01.09.2018, p. 58-66.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Proteomic analysis of Lactobacillus pentosus for the identification of potential markers of adhesion and other probiotic features

AU - Pérez Montoro, Beatriz

AU - Benomar, Nabil

AU - Gomez, Natacha Cabellero

AU - Ennahar, Said

AU - Horvatovich, Peter

AU - Knapp, Charles

AU - Alonso, Esther

AU - Gálvez, Antonio

AU - Abriouel, Hikmate

PY - 2018/9/1

Y1 - 2018/9/1

N2 - We analyzed the adhesion capacity to mucus of 31 Lactobacillus pentosus strains isolated from naturally fermented Aloreña green table olives using an immobilized mucin model. On the basis of their adhesive capacity to mucin, three phenotypes were selected for cell-wall protein proteomic analysis to pinpoint proteins involved in the adhesion process: the highly adhesive L. pentosus CF1-43 N (73.49% of adhesion ability), the moderately adhesive L. pentosus CF1-37 N (49.56% of adhesion ability) and the poorly adhesive L. pentosus CF2-20P (32.79% of adhesion ability). The results revealed four moonlighting proteins over-produced in the highly adhesive L. pentosus CF1-43 N, which were under/not produced in the other two L. pentosus strains (CF1-37 N and CF2-20P). These proteins were involved in glycolytic pathway (phosphoglycerate mutase and glucosamine-6-phosphate deaminase), stress response (small heat shock protein) and transcription (transcription elongation factor GreA). Furthermore, the relative fold change in gene expression analysis showed significant up-regulation of the genes coding for these four moonlighting proteins in the highly adhesive L. pentosus CF1-43 N versus the poorly adhesive L. pentosus CF2-20P and also in response to mucin for 20 h which clearly indicate the significant role of these genes in the adhesion capacity of L. pentosus. Thus, these proteins could be used as biomarkers for mucus adhesion in L. pentosus. On the other hand, mucin exposure induced other probiotic effects in L. pentosus strains, enhancing their co-aggregation ability with pathogens and possible inactivation.

AB - We analyzed the adhesion capacity to mucus of 31 Lactobacillus pentosus strains isolated from naturally fermented Aloreña green table olives using an immobilized mucin model. On the basis of their adhesive capacity to mucin, three phenotypes were selected for cell-wall protein proteomic analysis to pinpoint proteins involved in the adhesion process: the highly adhesive L. pentosus CF1-43 N (73.49% of adhesion ability), the moderately adhesive L. pentosus CF1-37 N (49.56% of adhesion ability) and the poorly adhesive L. pentosus CF2-20P (32.79% of adhesion ability). The results revealed four moonlighting proteins over-produced in the highly adhesive L. pentosus CF1-43 N, which were under/not produced in the other two L. pentosus strains (CF1-37 N and CF2-20P). These proteins were involved in glycolytic pathway (phosphoglycerate mutase and glucosamine-6-phosphate deaminase), stress response (small heat shock protein) and transcription (transcription elongation factor GreA). Furthermore, the relative fold change in gene expression analysis showed significant up-regulation of the genes coding for these four moonlighting proteins in the highly adhesive L. pentosus CF1-43 N versus the poorly adhesive L. pentosus CF2-20P and also in response to mucin for 20 h which clearly indicate the significant role of these genes in the adhesion capacity of L. pentosus. Thus, these proteins could be used as biomarkers for mucus adhesion in L. pentosus. On the other hand, mucin exposure induced other probiotic effects in L. pentosus strains, enhancing their co-aggregation ability with pathogens and possible inactivation.

KW - Lactobacillus pentosus

KW - proteomics

KW - probiotics

KW - mucus

KW - adhesion

KW - biomarkers

KW - qRT-PCR

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JO - Food Research International

JF - Food Research International

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ER -

Pérez Montoro B, Benomar N, Gomez NC, Ennahar S, Horvatovich P, Knapp C et al. Proteomic analysis of Lactobacillus pentosus for the identification of potential markers of adhesion and other probiotic features. Food Research International. 2018 Sep 1;111:58-66. https://doi.org/10.1016/j.foodres.2018.04.072

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